| 应用23S rRNA对禽波氏杆菌分离株的进化分析 |
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| 引用本文： | 谭燕玲,朱瑞良,马荣德,王慧,魏凯,王新建,郭文龙,朱明华.应用23S rRNA对禽波氏杆菌分离株的进化分析[J].中国兽医学报,2011,31(4). |
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| 作者姓名： | 谭燕玲 朱瑞良 马荣德 王慧 魏凯 王新建 郭文龙 朱明华 |
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| 作者单位： | 山东农业大学动物科技学院,山东泰安,271018 |
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| 基金项目： | 国家自然科学基金资助项目(30972183,30670114,30740077); 山东省自然科学基金资助项目(Y2006D09) |
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| 摘 要： | 通过PCR扩增分离保存的8株禽波氏杆菌、3株兔支气管败血波氏杆菌及1株猪支气管败血波氏杆菌菌株的23S rRNA基因的片段(710bp),分别克隆到载体pMD18-T后测序。利用BLAST工具进行同源性搜索;用DNAStar分析软件进行同源核苷酸序列的多重比较分析并构建禽波氏杆菌菌株的系统生物进化树。结果显示,8株禽波氏杆菌核苷酸序列之间同源性为99.2%～99.7%,与GenBank中收录的NC_010645株禽波氏杆菌核苷酸序列同源性为92.4%～92.5%,与兔支气管败血波氏杆菌和猪支气管败血波氏杆菌的核苷酸序列同源性均为98.5%～99.2%。国内分离的禽波氏杆菌菌株和支气管败血波氏杆菌菌株均与国外分离菌株在遗传基因上有一定的差异。结果表明,23S rRNA序列分析可以作为鉴定禽波氏杆菌的一种快速简便的方法。
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| 关 键 词： | 禽波氏杆菌 23S rRNA PCR 核苷酸序列 基因分析 |
Evolution analysis of 23S rRNA genes of Bordetella avium |
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| TAN Yan-ling,ZHU Rui-liang,MA Rong-de,WANG Hui,WEI Kai,WANG Xin-jian,GUO Wen-long,ZHU Ming-hua.Evolution analysis of 23S rRNA genes of Bordetella avium[J].Chinese Journal of Veterinary Science,2011,31(4). |
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| Authors: | TAN Yan-ling ZHU Rui-liang MA Rong-de WANG Hui WEI Kai WANG Xin-jian GUO Wen-long ZHU Ming-hua |
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| Institution: | TAN Yan-ling,ZHU Rui-liang*,MA Rong-de,WANG Hui,WEI Kai,WANG Xin-jian,GUO Wen-long,ZHU Ming-hua(College of Animal Science and Technology,Shandong Agricultural University,Tai'an,Shandong 271018,China) |
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| Abstract: | To assess the evolution of Bordetella avium by 23S rRNA genes.The fragments(710 bp) of 23S rRNA genes were amplified by polymerase chain reaction(PCR) from eight Bordetella avium strains,three rabbit Bordetella bronchiseptica strains and one porcine Bordetella bronchiseptica strain.The PCR products were cloned into plasmid vector pMD-18T and then sequenced.The nucleotides homology analysis of the 23S rRNA gene was carried out using the BLAST.Multiple sequences alignments and the phylogenetic tree were perfo... |
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| Keywords: | Bordetella avium 23S rRNA PCR nucleotide sequence gene analysis |
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