| 国产IBV疫苗株核衣壳蛋白基因的亲缘关系 |
| |
| 引用本文： | 曹伟胜,廖明,任涛,罗开健,辛朝安.国产IBV疫苗株核衣壳蛋白基因的亲缘关系[J].中国兽医学报,2003,23(2):119-123. |
| |
| 作者姓名： | 曹伟胜 廖明 任涛 罗开健 辛朝安 |
| |
| 作者单位： | 华南农业大学兽医学院,广东,广州,510642 |
| |
| 基金项目： | 国家自然科学基金重大项目 (3 9893 2 90 -2 ) |
| |
| 摘 要： | 利用自行设计的引物Cx和Cs，通过RT－PCR方法分别扩增出鸡传染性支气管炎病毒（IBV）D41株，H120GD株、H120SH株、H52GD株等4个国产疫苗株和标准强毒M41－E4株完整的N基因cDNA,然后将其分别克隆到pGEM T-Easy或pMD 18-T载体中并测序。测序结果表明，这5个IBV毒株可分2组，其中D41株，H120GD株和H120SH株为一组，它们的核苷酸和推导氨基酸序列同源性为99．7％－99．8％和99．0％－99．5％，而H52GD株与M41－E4株构成另一组，其核苷酸和推导氨基酸序列同源性为99．7％和99．5％；而2组之间的最大同源性仅为91．0％和93．2％。在系统发生进化树上，这2组分别位于不同的分支簇上。值得注意的是，国内的H52GD株与国外报道的H52株不在同一分支簇上，相反却与国内强毒M41－E4株以及国外报道的M41株在同一分支簇上。这一结果表明，国内的H52GD疫苗株与国外报道的H52疫苗株不同，它们在亲缘关系上更靠近M41－E4株和M41株。
|
| 关 键 词： | 核衣壳蛋白基因 亲缘关系 鸡 传染性支气管炎病毒 疫苗株 N基因 序列分析 |
| 文章编号： | 1005-4545(2003)02-0119-05 |
| 修稿时间： | 2001年11月21 |
Genetic Relationship of Nucleocapsid Gene of Domestic IBV Vaccine Strains |
| |
| CAO Wei-sheng,LIAO Ming,REN Tao,LUO Kai-jian,XIN Chao-an.Genetic Relationship of Nucleocapsid Gene of Domestic IBV Vaccine Strains[J].Chinese Journal of Veterinary Science,2003,23(2):119-123. |
| |
| Authors: | CAO Wei-sheng LIAO Ming REN Tao LUO Kai-jian XIN Chao-an |
| |
| Abstract: | The nucleocapsid protein gene(N gene) of IBV strain D41,H120GD,H120SH,H52GD and M41-E4 were amplified by RT-PCR with a pair of specific oligonucleotide primers(Cx/Cs) and cloned into plasmid pGEM-T Easy and pMD 18-T respectively.The inserts were sequenced and the results revealed that the complete N genes of strains D41,H120GD,H120SH,H52GD and M41-E4 were all composed of 1 230 bp,coding for 409 amino acids respectively.Sequence analysis revealed that the five IBV strains could be divided into two groups,i.e.,strains D41,H120GD and H120SH formed one group and there was 99.7%-99.8% nucleotide sequence homology and 99.0%-99.5% deduced amino acid sequence homology between strains D41,H120GD and H120SH;while strains H52GD and M41-E4 constituted another group and they had 99.7% nucleotide sequence homology and 99.5% deduced amino acid sequence homology each other.But the highest homology between the two groups was only 91.0% and 93.2%.The two groups fell into two different branches of a phylogenetic tree.It was noteworthy that domestic vaccine strain H52GD was not in the same branch with foreign H52 strain,but with domestic virulent M41-E4 strain and foreign M41 strain.The study suggested domestic H52 strain(H52GD) was different from foreign H52 strain because the former was more genetically related to domestic virulent M41-E4 strain and foreign M41 strain. |
| |
| Keywords: | infectious bronchitis virus vaccine strain nucleocapsid(N) gene sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
