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| 近交系大鼠DNA指纹图与微卫星DNA比较分析 | |
| 引用本文： | 李瑞生,王承利,赵林山,陈振文.近交系大鼠DNA指纹图与微卫星DNA比较分析[J].中国兽医学报,2003,23(3):297-299. |
| 作者姓名： | 李瑞生 王承利 赵林山 陈振文 |
| 作者单位： | 1. 军事医学科学院,实验动物中心,北京,100071 2. 沈阳军区总医院动物实验科,辽宁,沈阳,110015 3. 哈尔滨医科大学,实验动物中心,黑龙江,哈尔滨,150086 |
| 基金项目： | 总后勤部卫生部基金项目(98M116) |
| 摘 要： | 为了建立一种对近交系大鼠遗传物质进行精确可靠、快速简便的监测方法,利用DNA指纹技术对国内6个品系8个近交系大鼠群体进行了DNA多态性的分析,并与PCR扩增微卫星DNA技术进行了比较.其结果显示(1)不同品系之间DNA指纹图差异较大,同一群体不同个体间DNA指纹图带的相似系数和共有带率除SHR(哈)和WKY(哈)小于0.7外,其他均大于0.9.不同地区同一SHR间和WKY间DNA指纹图也存在差异,相同DNA不同次制作的DNA指纹图谱基本一致.(2)不同品系个体间微卫星DNA具有显著多态性;同一群体不同个体之间除SHR(哈)的SMST位点和WKY(哈)的AGT位点出现一定的差异外,其他均没有差异.DNA指纹图能更精确可靠地反映出动物个体间的遗传背景,而微卫星DNA遗传监测方法比较简便快捷. |
| 关 键 词： | 近交系大鼠 DNA指纹图 微卫星DNA |
| 文章编号： | 1005-4545(2003)03-0297-03 |
| 修稿时间： | 2002年1月12日 |
Comparison of DNA fingerprints of inbred rat strains with microsatellites DNA |
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| LI Rui sheng ,WANG Cheng li ,ZHAO Lin shan ,CHEN Zhen wen.Comparison of DNA fingerprints of inbred rat strains with microsatellites DNA[J].Chinese Journal of Veterinary Science,2003,23(3):297-299. | |
| Authors: | LI Rui sheng WANG Cheng li ZHAO Lin shan CHEN Zhen wen |
| Institution: | LI Rui sheng 1,WANG Cheng li 2,ZHAO Lin shan 3,CHEN Zhen wen 1* |
| Abstract: | In order to set up a exact,reliable,speedy method for monitoring the heredity of inbred rats,eight inbred rat groups of six strains were analyzed using DNA fingerprints.And compared with microsatellites DNA which was amplified by PCR.The results indicate:(1)There are great diversities between DNA fingerprints of various inbred strains rats.Excepting that the similarity coefficient and Band sharing level of SHR(Ha) and WKY(Ha) are less than 0 7 be tween the various individual of same group,the others are more than 0 9.There are diverses between the same inbred SHR strains or WKY strains in different areas.DNA fingerprints from the same DNA are almost coincident.(2)There are more polymorphism among the various inbred strain rats;and no polymorphism exists in the same inbred strain rats except SMST locus of SHR(Ha) and AGT locus of WKY(Ha).The method of DNA fingerprints would be a more exact,reliable for monitory the animal heredity. |
| Keywords: | inbred rats DNA fingerprints microsatellites DNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |