| South-Western blot mapping方法的建立及其应用于锥虫细胞核中DNA结合蛋白的观察 |
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| 引用本文： | 欧永春,T.Baltz.South-Western blot mapping方法的建立及其应用于锥虫细胞核中DNA结合蛋白的观察[J].中国兽医学报,1992(2). |
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| 作者姓名： | 欧永春 T.Baltz |
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| 作者单位： | 兽医大学军事兽医研究所
(欧永春),法国波尔多第二大学寄生虫免疫学和生物学实验室(T.Baltz) |
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| 摘 要： | South-Westerm blot mapping是一种结合Western blotting和Southern blotting某些特点的方法.本文介绍用其成功地观察到锥虫核蛋白中DNA结合蛋白的情况,并对一个分子量在40000左右、于较严谨条件下与DNA结合的核蛋白进行了特性鉴定.该蛋白等量地存在于锥虫的前循环期和血液期,对双链DNA有较大的亲和力,并能与酵母菌复制起始片段结合.本文还介绍了锥虫细胞核的提取技术和核蛋白的制备技术.
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| 关 键 词： | South-Western blot mapping DNA结合蛋白 基因组DNA 核蛋白 锥虫 |
Observation of DNA-binding Proteins from Trypanosome Nucle- ar Extracts with South-Western Blotting Mapping |
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| Ou Yongchun.Observation of DNA-binding Proteins from Trypanosome Nucle- ar Extracts with South-Western Blotting Mapping[J].Chinese Journal of Veterinary Science,1992(2). |
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| Authors: | Ou Yongchun |
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| Abstract: | A method which is referred to as "South-Western blotting mapping" is described. Nuclear extracts of trypanosomes are separated on SDS-polyacrylamide gel, blotted onto nitrocellulose by electrotransfer and hybridized with end-labelled DNA fragments. DNA-binding protein (proteins) is further characterized. The protein with Mr of 40000 is present in both blood stream and procyclic parasites. It has preferential affinity for the double-stranded DNA and binds yeast replication origin fragments at the same intensity as those of trypanosome genomic DNA. A method for preparation of nuclear proteins is also described. |
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| Keywords: | South-Western blotting mapping DNA-binding protein genomic DNA nu- clear proteins trypanosomes |
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