| 靶向GP5基因脱氧核酶抑制PRRSV在MARC-145细胞中的复制 |
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| 引用本文： | 宋德武,袁洁,李鑫,郭育培,杨建新,宣华,王贵平,丁壮.靶向GP5基因脱氧核酶抑制PRRSV在MARC-145细胞中的复制[J].中国兽医学报,2011,31(12). |
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| 作者姓名： | 宋德武 袁洁 李鑫 郭育培 杨建新 宣华 王贵平 丁壮 |
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| 作者单位： | 1. 吉林大学畜牧兽医学院,吉林长春,130062
2. 广东省农业科学院兽医研究所,广东广州,510640 |
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| 基金项目： | 广东省科技攻关重大项目(2008A020100020); 吉林大学研究生创新基金资助项目(20111077) |
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| 摘 要： | 针对猪繁殖与呼吸综合征病毒（PRRSV）JL/07/SW株GP5基因设计了4个脱氧核酶,经体外切割试验筛选得到具有体外切割活性的脱氧核酶,并通过RT-PCR、TCID50、CPE、间接免疫荧光（IFA）检测具有切割活性的脱氧核酶抑制PRRSV复制效果进行评价。结果针对GP5基因的Dz-GP5-10抑制效率最高可达到82.4%,Dz-GP5-12也表现良好的抑制效果,表明GP5基因的这2个位点可能是PRRSV复制所必需的。本研究为PRRSV复制及基因组功能研究、抗病毒药物开发和转基因动物研究奠定了基础。
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| 关 键 词： | PRRSV 脱氧核酶 GP5基因 |
Inhibition of PRRSV replication by DNAzymes targeted to matrix protein GP5 gene in MARC-145 cells |
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| SONG De-wu,YUAN Jie,LI Xin,GUO Yu-pei,YANG Jian-xin,XUAN Hua,WANG Gui-ping,DING Zhuang.Inhibition of PRRSV replication by DNAzymes targeted to matrix protein GP5 gene in MARC-145 cells[J].Chinese Journal of Veterinary Science,2011,31(12). |
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| Authors: | SONG De-wu YUAN Jie LI Xin GUO Yu-pei YANG Jian-xin XUAN Hua WANG Gui-ping DING Zhuang |
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| Institution: | SONG De-wu1,YUAN Jie2,LI Xin1,GUO Yu-pei1,YANG Jian-xin1,XUAN Hua2,WANG Gui-ping2,DING Zhuang1(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China,2.Institute of Veterinary Medicine,Guangdong Academy of Agricultural Science,Guangzhou 510640,China) |
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| Abstract: | Four DNAzymes targeting the porcine reproductive and respiratory syndrome virus(PRRSV) protein GP5 gene were designed.The active DNAzymes were derived by cleavage experiments in vitro.Marc145 cells were infected with PRRSV 6 h after the active DNAzymes were delivered,efficiency of DNAzymes was assayed by Real-time RT-PCR,TCID50,CPE and indirect immunofluorescence assay(IFA).This experiment successfully derived the active DNAzyme inhibition of PRRSV replication procedure in MARC-145 cells and showed that the... |
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| Keywords: | PRRSV DNAzyme GP5 gene |
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