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| 马链球菌兽疫亚种类M蛋白亚单位疫苗小鼠免疫试验 | |
| 引用本文： | 王建,刘佩红,沈素芳,沈莉萍,王永康,陆承平.马链球菌兽疫亚种类M蛋白亚单位疫苗小鼠免疫试验[J].中国兽医学报,2006,26(5):495-497. |
| 作者姓名： | 王建 刘佩红 沈素芳 沈莉萍 王永康 陆承平 |
| 作者单位： | 1. 上海市畜牧兽医站,上海,201103;南京农业大学,农业部畜禽疫病诊断与免疫重点实验室,江苏,南京,210095 2. 上海市畜牧兽医站,上海,201103 3. 南京农业大学,农业部畜禽疫病诊断与免疫重点实验室,江苏,南京,210095 |
| 基金项目： | 国家重点基础研究发展计划(973计划);上海市科技兴农科技攻关项目 |
| 摘 要： | 为获得控制猪链球菌病安全高效的疫苗，本试验进行了马链球菌兽疫亚种类M蛋白亚单位疫苗的研制。应用热酸法提取马链球菌兽疫亚种ATCC35246株类M蛋白，并分别采用羟基磷灰石（HAT）层析和冷酒精沉淀法进行纯化，结果得到较纯的类M蛋白。用热酸提取的粗制类M蛋白、2种纯化的类M蛋白及全菌苗免疫小鼠，结果保护率分别为92％（11／12）、100％（12／12）、100％（12／12）和83％（10／12），表明类M蛋白亚单位疫苗有进一步开发的必要。 |
| 关 键 词： | 马链球菌兽疫亚种 类M蛋白 疫苗 |
| 文章编号： | 1005-4545(2006)05-0495-03 |
| 收稿时间： | 2004-12-03 |
| 修稿时间： | 2004年12月3日 |
Test of M-like Protein Vaccine against Streptococcus equi subsp. zooepidemicus in Mice |
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| WANG Jian,LIU Pei-hong,SHEN Su-fang,SHEN Li-ping,WANG Yong-kang,LU Cheng-ping.Test of M-like Protein Vaccine against Streptococcus equi subsp. zooepidemicus in Mice[J].Chinese Journal of Veterinary Science,2006,26(5):495-497. | |
| Authors: | WANG Jian LIU Pei-hong SHEN Su-fang SHEN Li-ping WANG Yong-kang LU Cheng-ping |
| Institution: | 1. Shanghai Animal Husbandry and Veterinary Station ,Shanghai 201103 ,China ; 2. Key Laboratory of Animal Disease and Immunology,Ministry of Agriculture,Nanjing Agricultural University, Nanjing 210095 ,China |
| Abstract: | Streptococcus equi subsp.zooepidemicus,a lancifield group C Streptococcus,is an important pathogen of pigs.Previous studies have indicataed that M-like protein is an important virulence factor and protective antigen.We extracted the M-protein by hot-acid ways,and the acid-extracted proteins were partially purified by hydroxyapatite(HAT) chromatography and cold-alcohol precipitate.The whole cell bacterin,the acid-extracted protein and partially purified M-like protein were applied to vaccinate mice with the protective efficacy of 83.3%(10/12),92%(11/12),100%(12/12) respectively.It suggested that the M-like protein vaccine against Streptococcus equi subsp.zooepidemicus would be necessary for to be further developed. |
| Keywords: | Streptococcus equi M-like protein vaccine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |