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| 黄曲霉毒素B1免疫层析检测方法的研究 | |
| 引用本文： | 许远靖,于师宇,田武林,孙勇,王冲,白鸽,肖成蕊,牟俊,于光,谢光洪,王哲.黄曲霉毒素B1免疫层析检测方法的研究[J].中国兽医学报,2012,32(6):894-897. |
| 作者姓名： | 许远靖 于师宇 田武林 孙勇 王冲 白鸽 肖成蕊 牟俊 于光 谢光洪 王哲 |
| 作者单位： | 1. 吉林大学畜牧兽医学院,吉林长春,130062 2. 吉林大学畜牧兽医学院,吉林长春130062／福建出入境检验检疫局,福建福州350001 3. 吉林出入境检验检疫局,吉林长春,130062 |
| 基金项目： | 国家质检总局科技计划项目(2005J0088) |
| 摘 要： | 以柠檬酸三钠为还原剂，制备了20nm胶体金颗粒，以此胶体金标记纯化的单抗4G6，喷于玻璃纤维上，AFBl蛋白偶联物和羊抗鼠IgG分别结合于硝酸纤维膜上，依次将样本垫、胶金垫、硝酸纤维膜和吸水纸组装，切割成胶体金试纸条。结果：组装的试纸条特异性好，与AF类似物无交叉反应，对AFBl标准品最低检测限为3μg／L，检测时间约为10min，批内和批间重复性为100％，假阳性率和假阴性率均为0。操作简便，成本很低，适于AFBl的现场快速检测。在制备了单克隆抗体的基础上，应用胶体金免疫层析技术，建立了食品中黄曲霉毒素B1（Aflatoxin B1，AFBl）快速检测方法。 |
| 关 键 词： | 黄曲霉毒素B1 胶体金免疫层析 试纸条 快速检测 |
Study on colloidal gold immunochromatography assay for rapid detection of aflatoxin B1 |
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| XU Yuan-jing,YU Shi-yuan,TIAN Wu-lin,SUN Yong,WANG Chong,BAI Ge,XIAO Cheng-rui,MU Jun,YU Guang,XIE Guang-hong,WANG Zhe.Study on colloidal gold immunochromatography assay for rapid detection of aflatoxin B1[J].Chinese Journal of Veterinary Science,2012,32(6):894-897. | |
| Authors: | XU Yuan-jing YU Shi-yuan TIAN Wu-lin SUN Yong WANG Chong BAI Ge XIAO Cheng-rui MU Jun YU Guang XIE Guang-hong WANG Zhe |
| Institution: | 1(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.Jilin Entry-Exit Inspection and Quarantine Bureau,Changchun 130062,China;3.Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou 350001,China) |
| Abstract: | Colloidal gold immunity chromotography technique was applied in this research.A rapid detection method for aflatoxin B1 in food was established.The method of trisodium citrate disoxidation was used to prepare colloidal gold particles which were used to mark AFB1 monoclonal antibodies and sprayed on the fiberglass.AFB1-protein and peroxidase-conjugated goat anti-mouse IgG combine on nitrocellulose independently,then sample tray,colloidal gold tray,nitrocellulose and bibulous paper were assembled to form colloidal gold test paper ti form the detection card.The result indicated the sensitivity of AFB1 fast detecting test paper was 45 μg/L;testing time was 10 min;the reproducibility between group inner and group exterior was 100%;false positive rate and false negative rate were all 0.It’s simple and convenient for fast detecting of AFB1 in food on the spot. |
| Keywords: | aflatoxin B1 colloidal gold immunity chromotography test paper fast detecting |
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