| 猕猴源马链球菌兽疫亚种的分离鉴定 |
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| 引用本文： | 程龙飞,吴志强,刘荣昌,孔丽芳,傅光华,陈红梅,黄瑜.猕猴源马链球菌兽疫亚种的分离鉴定[J].中国兽医学报,2020(6):1158-1161. |
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| 作者姓名： | 程龙飞 吴志强 刘荣昌 孔丽芳 傅光华 陈红梅 黄瑜 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所;南平市城市公园管理处 |
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| 基金项目： | 国家百千万人才补助基金资助项目(NCNCMTPC-2009);福建省百千万领军人才资助项目(FJTMTP-2014)。 |
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| 摘 要： | 为鉴定死亡猕猴血液中分离的细菌,本试验采用16S rRNA序列比对的方法对该菌进行鉴定。药敏试验采用纸片法,毒力试验采用皮下注射小鼠的方法。结果显示,所鉴定细菌为马链球菌兽疫亚种,敏感的药物有氨苄青霉素、阿莫西林、头孢唑啉、克林霉素、利福平、氟苯尼考、红霉素和罗红霉素等。经皮下注射700个活菌即能致死小鼠。结果表明,南平某动物园此次猕猴死亡是由马链球菌兽疫亚种引起的。
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| 关 键 词： | 猕猴 马链球菌兽疫亚种 16S rRNA 药物敏感性 |
Isolation and identification of S.equi ssp. zooepidemicus from Macaca mulatta |
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| CHENG Long-fei,WU Zhi-qiang,LIU Rong-chang,KONG Li-fang,FU Guang-hua,CHEN Hong-mei,HUANG Yu.Isolation and identification of S.equi ssp. zooepidemicus from Macaca mulatta[J].Chinese Journal of Veterinary Science,2020(6):1158-1161. |
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| Authors: | CHENG Long-fei WU Zhi-qiang LIU Rong-chang KONG Li-fang FU Guang-hua CHEN Hong-mei HUANG Yu |
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| Institution: | (Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;City Park Management Office of Nanping City,Nanping,Fujian 353000,China) |
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| Abstract: | To identify the bacteria isolated from blood of dead Macaca mulatta.The bacteria were identified by 16 S rRNA sequence alignment,and drug sensitivity by diffusion test on paper,toxicity by subcutaneous injection of mice.The strain of bacteria was identified as S.equi ssp.zooepidemicus.It was sensitive to ampicillin,amoxicillin,cefazolin,clindamycin,rifampicin,florfenicol,erythromycin and roxithromycin.Mice could be killed by subcutaneous injection of 700 CFU.The results showed that the Macaca mulatta death in a zoo in Nanping was caused by S.equi ssp.zooepidemicus. |
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| Keywords: | Macaca mulatta S equi ssp zooepidemicus 16S rRNA drug sensitivity |
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