| F-2毒素致体外培养大鼠睾丸支持细胞DNA损伤 |
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| 引用本文： | 邬静,袁莉芸,袁慧.F-2毒素致体外培养大鼠睾丸支持细胞DNA损伤[J].中国兽医学报,2007,27(5):731-732,736. |
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| 作者姓名： | 邬静 袁莉芸 袁慧 |
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| 作者单位： | 湖南农业大学,动物医学学院,湖南,长沙,410128 |
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| 摘 要： | 为探讨F-2毒素对雄性生殖机能的影响,取大鼠睾丸支持细胞进行体外培养,运用单细胞凝胶电泳技术检测51、0、20和40 mg/L的F-2毒素攻毒后24 h支持细胞DNA的损伤情况。结果显示,F-2毒素在一定浓度范围内对体外培养的支持细胞DNA产生损伤作用,且受损程度随着F-2毒素攻毒剂量而升高,具有明显量效关系。除5 mg/L剂量组外,其余各组的细胞受损率、彗星尾长和DNA损伤程度与阴性对照组相比差异极显著（P〈0.01）,表明F-2毒素对体外培养的大鼠睾丸支持细胞有毒性作用,能够损伤细胞DNA。
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| 关 键 词： | 大鼠 F-2毒素 支持细胞 单细胞凝胶电泳 DNA损伤 |
| 文章编号： | 1005-4545（2007）05-0731-02 |
| 修稿时间： | 2005-12-21 |
DNA damage of rat testicle sertoli cell in vitro caused by F-2 toxin |
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| WU Jing,YUAN Li-yun,YUAN Hui.DNA damage of rat testicle sertoli cell in vitro caused by F-2 toxin[J].Chinese Journal of Veterinary Science,2007,27(5):731-732,736. |
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| Authors: | WU Jing YUAN Li-yun YUAN Hui |
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| Institution: | College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China |
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| Abstract: | To explore the effects of F-2 toxin on male reproduction function,the rat testicle sertoli cell cultures were exposed to F-2 toxin within a dose range of 5-40 mg/L,the DNA damage during 24 h was determined by single-cell gel eleetrophoresis(SCGE).The results showed that F-2 toxin led to the damage on DNA of sertoli cells within the definite range of concentration.The extent of damage increased with the concentration,suggesting an evident dose-effect relationship.And except 5 mg/L group,the difference of the tail rate,average tail length and DNA damage in each group was extremely remarkable as compared with negative group(P<0.01).These results indicated that F-2 toxin can cause DNA damage of the rat testicle sertoli cell. |
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| Keywords: | rat F-2 toxin sertoli cells SCGE DNA damage |
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