| 研究不同氨基酸模式对奶牛乳腺上皮细胞生长的影响 |
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| 引用本文： | 陈智梅,高民,刘桂瑞,王瑶,李正洪,李兆林.研究不同氨基酸模式对奶牛乳腺上皮细胞生长的影响[J].中国奶牛,2010(11):4-6. |
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| 作者姓名： | 陈智梅 高民 刘桂瑞 王瑶 李正洪 李兆林 |
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| 作者单位： | [1]内蒙古奶联科技有限公司,呼和浩特010050 [2]内蒙古农牧业科学院动物营养研究所,呼和浩特010030 |
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| 摘 要： | 本试验以泌乳奶牛乳腺组织为原料,采用组织块培养技术,研究培养基内不同氨基酸模式对奶牛乳腺上皮细胞增殖的影响。结果表明,随着培养天数的增加,细胞数量逐渐增加,第10天各组细胞数量分别达到最高,乳腺上皮细胞生长曲线呈"S"形。不同氨基酸模式对乳腺细胞生长有显著影响,与氨基酸不平衡组相比,氨基酸平衡组、理想组更能促进细胞生长,各组间差异显著(P0.05)。
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| 关 键 词： | 奶牛 乳腺上皮细胞 氨基酸模式 培养 生长 |
The Effects of Different Amino Acid Pattern on Proliferation of Mammary Epithelial Cells in Cows |
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| CHEN Zhi-mei,GAO Min,LIU Gui-rui,WANG Yao,LI Zheng-hong,LI Zhao-lin.The Effects of Different Amino Acid Pattern on Proliferation of Mammary Epithelial Cells in Cows[J].China Dairy Cattle,2010(11):4-6. |
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| Authors: | CHEN Zhi-mei GAO Min LIU Gui-rui WANG Yao LI Zheng-hong LI Zhao-lin |
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| Institution: | 1.Inner Mongolia Dairyunited Sci-Tech Co.,Ltd,Huhhot 010050;2.Animal Nutrition Institue,Inner Mongolian Academy of Agriculture and Animal Science,Huhhot 010018) |
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| Abstract: | The effects of different amino acid pattern on proli-feration of mammary epithelial cells of dairy cattle,cell growth of mammary epithelial cells were studied in this paper.The results showed that：while the number of cultured days increased,the number of cells increasd.The number of cells increased to its maximum on the tenth day and declined afterward.Mammary epithelial cell growth curve ＂S＂ shape.The different amino acid pattern had significant impact on cell growth of mammary epithelial cells.Compared with the amino acid imbalance,amino acid balance,the ideal group to better promote cell growth.The differences between the groups were significant（P0.05）. |
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| Keywords: | Dairy cows mammary epithelial cells Amino acid Pattern Culture Growth |
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