| 紫花苜蓿中芦丁的提取及抗菌活性研究 |
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| 引用本文： | 赵强,赵海福.紫花苜蓿中芦丁的提取及抗菌活性研究[J].中国奶牛,2013(20):25-27. |
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| 作者姓名： | 赵强 赵海福 |
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| 作者单位： | [1]中国科学院西北高原生物研究所,西宁810001 [2]甘肃农业大学动物医学院,兰州730070 |
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| 基金项目： | 甘肃省技术研究与开发专项计划（0912TCYL004）基金资助. |
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| 摘 要： | 本研究通过正交试验优选了紫花苜蓿中芦丁的提取工艺,以510nm最大吸光度进行可见光谱检测,测定芦丁含量,并对两种供试菌进行了体外抑菌活性测定.结果表明,通过单因素试验确定的各因素影响能力大小为：料液比＞乙醇浓度＞超声提取时间,含量最高为10.04mg/g;大肠埃希氏杆菌的MIC为0.40mg/mL,金黄色葡萄球菌的MIC为0.70mg/mL.
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| 关 键 词： | 紫花苜蓿 芦丁 抗菌 |
Study on Extraction and Antibacterial of Rutin from Alfalfa |
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| ZHAO Qiang;ZHAO Hai-fu.Study on Extraction and Antibacterial of Rutin from Alfalfa[J].China Dairy Cattle,2013(20):25-27. |
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| Authors: | ZHAO Qiang;ZHAO Hai-fu |
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| Institution: | ZHAO Qiang;ZHAO Hai-fu(Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, Xining 810001;College of Vetetinary Medicine at Gansu Agricaltural Univesity, Lanzhou 730070) |
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| Abstract: | The extraction method of rutin from alfalfa was selected through an orthogonal test,the content determination of rutin was test 510 nm maximum spectrophotometry visible spectrum and the antibacterial activity for two selected bacteria in vitro was determination.The results showed that the order for the influence of various factors was Liquid to solid ratio ＞ Ethanol concentration ＞ Ultrasonic extraction time,the highest content was 10.04 mg/g,the MIC for E.coli and S.aureus were 0.40 mg/mL and 0.70 mg/mL respectively. |
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| Keywords: | Alfalfa Rutin Antibacterial |
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