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| H9N2亚型禽流感病毒陕西分离株的鉴定及HA、NP、NA全序列测定 | |
| 引用本文： | 曹维伟,陈杰,张燕霞,黄保续,洪军,陈德坤,李晓成.H9N2亚型禽流感病毒陕西分离株的鉴定及HA、NP、NA全序列测定[J].中国动物检疫,2006,23(1):25-27,43. |
| 作者姓名： | 曹维伟 陈杰 张燕霞 黄保续 洪军 陈德坤 李晓成 |
| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西,杨凌,712100;农业部动物检疫所国家动物流行病学中心,山东,青岛,266032 2. 农业部动物检疫所国家动物流行病学中心,山东,青岛,266032 3. 西北农林科技大学动物科技学院,陕西,杨凌,712100 |
| 摘 要： | 从陕西地区疑似流感发病鸡分离到4株流感病毒,经国家流感中心鉴定均为A型流感病毒H9N2亚型。将其中1株A/Chicken/Shaanxi/3/2002(H9N2)的血凝素基因核蛋白基因和神经氨酸酶基因进行克隆和测序,与GenBank收录的其它流感H9N2亚型的相关基因进行比较,结果表明,A/Chicken/Shaanxi/3/2002(H9N2)的NA序列与A/Chicken/Shanghai/F/98(H9N2)的NA序列同源性较高,为98.9%,但其HA序列与香港人流感病毒A/HongKong/1073/99、A/HongKong/1074/99(H9N2)的HA序列同源性较低,为85.9%。另外,氨基酸进化树分析结果显示,A/Chicken/Shaanxi/3/2002(H9N2)与A/chicken/HongKong/CSW153/03(H9N2)的亲缘关系最近,两者形成独立的分支。 |
| 关 键 词： | H9N2亚型 禽流感病毒 HA NP NA 序列比较 |
| 文章编号： | 1005-944X（2006）01-0025-04 |
Isolation and Identification of Subtype H9N2 Avian Influenza Virus from Shaanxi Province and Sequence Analysis of It's HA、NP、NA Gene |
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| Cao Weiwei,Chen Jie,Zhang Yanxi,Huang Baoxu,Hong jun,Chen Dekun,Li Xiaocheng.Isolation and Identification of Subtype H9N2 Avian Influenza Virus from Shaanxi Province and Sequence Analysis of It''''s HA、NP、NA Gene[J].China Journal Of Animal Quarantine,2006,23(1):25-27,43. | |
| Authors: | Cao Weiwei Chen Jie Zhang Yanxi Huang Baoxu Hong jun Chen Dekun Li Xiaocheng |
| Institution: | 1.Animal Science Academy, Northwest Sci-tech University of Agriculture and Forestry, Yangling Shanxi 712100;2. China Epizootiology Centre, Animal Quarantine Institute of MOA Qingdao Shandong 266032 |
| Abstract: | Four strains of avian influenza virus were isolated from sick chickens in different farms of Shaanxi province from May 1998 to May 2003.They were all identified as influenza A virus of H9N2 subtype by National Influenza Research Center. The complete HA?NP?NA gene of one isolate A/Chicken/Shaanxi/3/2002(H9N2) was cloned,sequenced and compared with sequences available in GenBank. The results indicated that the isolate A/Chicken/Shaanxi/3/2002(H9N2)possibly came from A/Chicken/Shanghai/F/98. |
| Keywords: | H9N2 subtype avian influenza virus HA NP NA gene sequence |
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