| 对虾急性肝胰腺坏死综合征不同检测方法结果分析 |
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| 引用本文： | 郭书林,尤颖哲,陈何东,王艺红,陈信忠,丁亦男,龚艳清,杨俊萍,陈长乐,陈炳颖.对虾急性肝胰腺坏死综合征不同检测方法结果分析[J].中国动物检疫,2020,37(1):87-90. |
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| 作者姓名： | 郭书林 尤颖哲 陈何东 王艺红 陈信忠 丁亦男 龚艳清 杨俊萍 陈长乐 陈炳颖 |
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| 作者单位： | 厦门海关;漳州市水产技术推广站 |
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| 基金项目： | 福建省科技计划项目（2018N0031） |
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| 摘 要： | 急性肝胰腺坏死综合征(acute hepatopancreas necrosis disease,AHPND)是一种危害大、新出现的对虾疫病。目前认为该病由一种副溶血性弧菌(Vibrio parahaemolyticus,VP)引起。本试验通过生化鉴定、血清学分型、MALDI-TOF-MS鉴定以及文献建立的PCR方法,在福建省南美白对虾养殖场,对分离于AHPND病虾肝胰腺的VP进行了检测。通过VITEK-2鉴定出81株VP分离株。从中选取9株分别进行MALDI-TOF-MS鉴定、PCR检测和血清学分型,发现这9株分离菌株均与MALDI-TOF-MS数据库中的VP匹配。其中:4株PCR检测为阳性,被鉴定为AHPND VP,其血清型包括O1:KUT和O1:K68;另5株PCR检测为阴性,血清型包括O1:KUT、O3:K6和O1:K68。试验表明:AHPND VP分离株存在不同血清型,可通过MALDI-TOF-MS进行菌种鉴定;MALDI-TOF-MS与PCR结合使用,可以准确、快速鉴定AHPND VP,这有利于开展AHPND的病原学分析、流行病学调查以及诊断和监测。
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| 关 键 词： | 急性肝胰腺坏死综合症 副溶血性弧菌 MALDI-TOF-MS PCR 血清型 鉴定 |
Analysis on the Results of Different Detection Methods for AHPND in Shrimps |
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| Guo Shulin,You Yingzhe,Chen Hedong,Wang Yihong,Chen Xinzhong,Ding Yinan,Gong Yanqing,Yang Junping,ChenChangle,Chen Bingying.Analysis on the Results of Different Detection Methods for AHPND in Shrimps[J].China Journal Of Animal Quarantine,2020,37(1):87-90. |
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| Authors: | Guo Shulin You Yingzhe Chen Hedong Wang Yihong Chen Xinzhong Ding Yinan Gong Yanqing Yang Junping ChenChangle Chen Bingying |
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| Institution: | (Xiamen Customs,Xiamen,Fujian 361016,China;Zhangzhou Fishery Technology Extension Station,Zhangzhou,Fujian 363000,China) |
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| Abstract: | At present,acute hepatopancreatic necrosis disease(AHPND),a harmful and emerging shrimps disease,was considered to be caused by Vibrio parahaemolyticus(VP).In this study,the VP strains isolated from the hepatopancreas of shrimps infected with AHPND in a white shrimp farm in Fujian Province were tested by biochemical identification,serological typing,MALDI-TOF-MS and the PCR assay published in related literature.A total of 81 VP isolates were confirmed by VITEK-2,from which,9 strains were selected for MALDI-TOF-MS identification,serological typing and PCR assay,which turned out to be compatible with the VP in MALDI-TOF-MS database.Specifically,according to results of PCR assay,4 strains were detected positive and identified as AHPND VP,and their serotypes were O1:KUT and O1:K68;the other 5 strains were negative with the serotypes of O1:KUT,O3:K6 and O1:K68.In conclusion,there were different serotypes in AHPND VP strains that could be identified by MALDI-TOF-MS;the AHPND VP could be identified accurately and rapidly by combination of MALDI-TOF-MS and PCR assay,which contributed to pathogenic analysis,epidemiological investigation,diagnosis and monitoring against the disease. |
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| Keywords: | AHPND AHPND Vibrio parahemolyticus MALDI-TOF-MS PCR serotype identi cation |
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