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| 奶牛猝死病例中O101大肠杆菌的分离及部分毒力相关抗原的鉴定 | |
| 引用本文： | 倪宏波,孙福先,王冰,石星明,姜海芳,周玉龙,王春仁,宫大庆.奶牛猝死病例中O101大肠杆菌的分离及部分毒力相关抗原的鉴定[J].中国预防兽医学报,2011,33(3). |
| 作者姓名： | 倪宏波 孙福先 王冰 石星明 姜海芳 周玉龙 王春仁 宫大庆 |
| 作者单位： | 1. 黑龙江八一农垦大学,动物科技学院,黑龙江大庆163319 2. 黑龙江省农垦总局畜牧兽医局,黑龙江哈尔滨,150036 3. 黑龙江农垦职业学院食品工程系,黑龙江,哈尔滨150025 4. 中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨,150001 |
| 基金项目： | 黑龙江省科技厅实验动物青年基金项目(PC07S09) |
| 摘 要： | 为鉴定引起奶牛猝死综合征的病原及其毒力相关抗原,本研究无菌采集急性死亡奶牛的肝、脾、肾及小肠内容物样本,进行病原菌的分离和纯化,通过微生物学诊断、肠毒素试验、血清学试验、药敏试验和菌毛PCR进行鉴定,结果表明所分离的菌株为产ST肠毒素并具有F41菌毛、血清型为O101的大肠杆菌,该菌株对克林霉素、多黏菌素B、头孢曲松高度敏感。本实验为进行奶牛大肠杆菌性猝死综合征的防治奠定了基础。 |
| 关 键 词： | 猝死综合症 肠毒素 血清型 菌毛 |
Isolation and identification of pathogenic bacteria and virulence related antigen from bovine sudden-death syndrome |
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| NI Hong-bo,SUN Fu-xian,WANG Bing,SHI Xing-ming,JIANG Hai-fang,ZHOU Yu-long,WANG Chun-ren,GONG Da-qing.Isolation and identification of pathogenic bacteria and virulence related antigen from bovine sudden-death syndrome[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(3). | |
| Authors: | NI Hong-bo SUN Fu-xian WANG Bing SHI Xing-ming JIANG Hai-fang ZHOU Yu-long WANG Chun-ren GONG Da-qing |
| Institution: | NI Hong-bo1,SUN Fu-xian2,WANG Bing3,SHI Xing-ming4,JIANG Hai-fang1,ZHOU Yu-long1,WANG Chun-ren1,GONG Da-qing1(1.College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China,2.Animal Husbandry and Veterinary Division of Heilongjiang Rectamation Bureau,Harbin 150036,3.Department of Food Engineering,Heilongjiang Land Reclamation Areer College,Harbin 150025,4.Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
| Abstract: | To identify the pathogen of Bovine Sudden death Syndrome(BSDS) and its virulence related antigens.The liver,spleen,kidney and small intestine contents from the death dairy cow were collected under aseptic conditions,the pathogenic bacteria was isolated and identified by the microbiological diagnosis,enterotoxin test,serology test,drug sensitive test and PCR for the pilus encoding gene.The result indicated that the isolate was E.coli serogroup O101 which produced the ST enterotoxin and contain the F41 pilus.... |
| Keywords: | sudden death syndrome enterotoxin serotype pilus |
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