| 6株副猪嗜血杆菌基因组DNA的PCR指纹图谱研究 |
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| 引用本文： | 朱必凤,杨旭夫,刘主,彭凌,邓小亮.6株副猪嗜血杆菌基因组DNA的PCR指纹图谱研究[J].中国预防兽医学报,2007,29(3):177-179,184. |
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| 作者姓名： | 朱必凤 杨旭夫 刘主 彭凌 邓小亮 |
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| 作者单位： | 韶关学院,英东生物工程学院,广东,韶关,512005 |
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| 基金项目： | 广东省科技攻关计划;霍英东铭源基金 |
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| 摘 要： | 根据肠道菌基因闻重复一致序列，设计了一对特异性引物，采用ERIC-PCR和RAPD技术，研究了副猪嗜血杆菌6个分离菌株的指纹图谱和DNA多态性。结果表明，6个分离株的PCR指纹图谱与15个标准血清型指纹图谱相比较可分辨出4种血清型；6个分离株的RAPD研究结果均表现出多态性。有意义的是，6个菌株的多态性DNA片段也能明显将其分为4种类型的副猪嗜血杆菌，与特异性引物PCR结果相一致。该研究可作为流行病学调查和该菌的分子分型快速诊断方法的基础。
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| 关 键 词： | 副猪嗜血杆菌 指纹图谱 分子分型 |
| 文章编号： | 1008-0589（2007）03-177-03 |
| 收稿时间： | 2006-05-26 |
| 修稿时间： | 2006年5月26日 |
PCR fingerprints of genomes DNA of six strains Haemophilus parasuis |
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| ZHU Bi-feng,YANG Xu-fu,LIU Zhu,PENG Ling,DENG Xiao-liang.PCR fingerprints of genomes DNA of six strains Haemophilus parasuis[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(3):177-179,184. |
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| Authors: | ZHU Bi-feng YANG Xu-fu LIU Zhu PENG Ling DENG Xiao-liang |
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| Abstract: | A pair of specific primers was designed based on enterobacterial repetitive intergenic consensus(ERIC) sequence.The DNA fingerprints of 6 isolates of H.parasuis(HPS) from hoggery were investigated by ERIC-PCR.The polymorphism of the HPS strains was analyzed by PCR with random primers.By comparing to the fingerprints of 15 serotypes of H.parasuis,the 6 isolates were identified as four serotypes.Interestingly,the result of polymorphism analysis could also differentiate the 6 isolates into four groups,which is consistent with the results obtained from the specific primers.The method could be useful for study epidemiology and molecular typing of H.parasuis. |
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| Keywords: | ERIC-PCR RAPD |
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