| 貂源肺炎克雷伯菌的分离与鉴定 |
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| 引用本文： | 胡本钢,杜崇涛,雷连成,韩文瑜.貂源肺炎克雷伯菌的分离与鉴定[J].中国预防兽医学报,2011,33(8). |
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| 作者姓名： | 胡本钢 杜崇涛 雷连成 韩文瑜 |
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| 作者单位： | 吉林大学畜牧兽医学院,吉林长春,130062 |
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| 基金项目： | 高等学校博士学科点专项科研基金(101082261029); 吉林大学基本科研业务费(421030300101) |
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| 摘 要： | 本研究从疑似感染肺炎克雷伯菌(Kpn)的病死水貂病料中分离得到5株革兰氏阴性杆菌,通过对其进行细菌形态学、培养特征、生化特性和分子生物学鉴定,确认分离得到的菌株为Kpn。16S rRNA基因序列分析结果显示:该分离菌株与已知的Kpn相应序列的同源性为100%;药敏试验显示分离株对头孢曲松和舒巴坦高度敏感。动物致病性试验显示该分离株对小鼠具有较强的致病性。
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| 关 键 词： | 肺炎克雷伯菌 分离鉴定 16S rRNA |
Isolation and identification of Klebsiella pneumoniae from mink |
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| HU Ben-gang,DU Chong-tao,LEI Lian-cheng,HAN Wen-yu.Isolation and identification of Klebsiella pneumoniae from mink[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(8). |
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| Authors: | HU Ben-gang DU Chong-tao LEI Lian-cheng HAN Wen-yu |
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| Institution: | HU Ben-gang,DU Chong-tao,LEI Lian-cheng*,HAN Wen-yu(College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China) |
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| Abstract: | Five isolates of gram-nagative bacteria were isolated from affected mink and identified as Klebsiella pneumoniae by bacteria morphology,cultural character,physiological,biochemical characteristics and 16S rRNA sequencing.The result revealed that 16S rRNA gene of these isolates shared the homology of 100% with reference strain of K.pneumoniae.Drug sensitive tests showed that these isolates were highly sensitive to ceftriaxone and sulbactam.Experimeutal infection test indicated that the bacterium had high pat... |
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| Keywords: | Klebsiella pneumoniae isolation and identification 16S rRNA |
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