| 马流感病毒A/马/青海1/94株亚型鉴定及其HA基因序列特征 |
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| 引用本文： | 王晓钧,贾斌,杨建德,田国斌,薛飞,唐秀英,相文华,沈荣显.马流感病毒A/马/青海1/94株亚型鉴定及其HA基因序列特征[J].中国预防兽医学报,2003,25(3):164-167. |
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| 作者姓名： | 王晓钧 贾斌 杨建德 田国斌 薛飞 唐秀英 相文华 沈荣显 |
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| 作者单位： | 中国农业科学院,哈尔滨兽医研究所,黑龙江,哈尔滨,150001 |
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| 基金项目： | 国家自然科学基金资助项目 (395 70 5 37# ) |
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| 摘 要： | 本试验用AI标准阳性分型血清对我国马流感病毒A／马/青海/1／94进行了血凝素(H)和神经氨酸酶(N)的鉴定，并与马流感病毒吉林株、黑龙江株、北京株及国际标准株A／切ukx／Mgxd／2／63进行了对比，结果显示，1944年在我国青海省暴发的马流感病毒的亚型为H3N8；以反转录。聚合酶链式反应(RT-PCR)扩增该株的血凝素基因，并克隆到pGEM-Teasy载体上，采用双脱氧末端终止法测定该cDNA片段共1738个核苷酸序列，并推导出其编码的565个氨基酸的序列。利用Genbank blast和基因进化树分析软件分析各毒株之间的亲缘关系，发现A／马/青海/1/94与1992年香港马流感分离株存在较近的亲缘关系。
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| 关 键 词： | 马流感病毒 亚型鉴定 血凝素基因 |
| 文章编号： | 1008-0589(2003)03-00164-04 |
| 修稿时间： | 2002年9月17日 |
Subtype determination and characters of HA gene of equine influenza virus qinghai strain |
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| WANG Xiao_jun,JIA Bin,YANG Jian_de,TIAN Guo_bin,XUE Fei,TANG Xiu_ying,XIANG Wen_hua,SHEN Rong_xian.Subtype determination and characters of HA gene of equine influenza virus qinghai strain[J].Chinese Journal of Preventive Veterinary Medicine,2003,25(3):164-167. |
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| Authors: | WANG Xiao_jun JIA Bin YANG Jian_de TIAN Guo_bin XUE Fei TANG Xiu_ying XIANG Wen_hua SHEN Rong_xian |
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| Abstract: | The subtype of a equine influenza virus strain isolated from west China,A/Equine/QingHai/1/94,was determined by using the standard sera of avian influenza virus and was compared with the subtypes of A/Equine/JiLin/2/89,A/Equine/HeiLongjiang/2/89,A/Equine/BeiJing/1/74,as well as the international standard strain,A/Equine/Maimi/2/63.The result showed that the subtype of the A/Equine/QingHai/1/94 was H 3N 8.The hemagglutinins gene was amplifed by RT_PCR and cloned into pGEM_T easy vetor.A total 1738 base pairs of hemagglutinins gene were sequenced and analysed by computer software.The results of blast and phyligenetic tree analysis showed that the HA gene of A/Equine/Qinghai/1/94/was very near to A/Equine/HongKong/1/92.This date indicates that the epidemic of equine influenza in Qinghai province in 1994 has a close relationship with the avian influenza outbreak in HongKong in 1992. |
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| Keywords: | equine influenza virus subtype determination HA gene |
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