| 鸭圆环病毒ORF3基因的克隆及序列分析 |
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| 引用本文： | 魏宗,邹金峰,雷战,孙慧,王鑫,相琪旺,赵钦,姜世金.鸭圆环病毒ORF3基因的克隆及序列分析[J].中国预防兽医学报,2011,33(9). |
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| 作者姓名： | 魏宗 邹金峰 雷战 孙慧 王鑫 相琪旺 赵钦 姜世金 |
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| 作者单位： | 1. 山东农业大学动物医学院,山东泰安,271018
2. 山东省动物生物工程与疾病防治重点实验室,山东泰安,271018 |
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| 基金项目： | 山东省科技发展计划项目(2010GNC10914) |
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| 摘 要： | 为了解鸭圆环病毒(DuCV)不同分离株ORF3基因变异情况及分析其同源性,本研究从临床病料中分离10株DuCV,扩增ORF3基因并测定其序列.通过与已知序列比对分析发现,DuCV ORF3基因分为两种基因型,大小分别为237 bp和297bp.ORF3基因同一基因型之间的核苷酸同源性为95.8％～100％,氨基酸同源性为88.6％～100％,而不同基因型之间的核苷酸同源性仅为87.8％～91.6％,氨基酸同源性仅为72.2％～81.0％.
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| 关 键 词： | 鸭圆环病毒 ORF3基因 序列分析 基因型 |
Cloning and sequence analysis of ORF3 gene of duck circovirus |
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| WEI Zong,ZOU Jin-feng,LEI Zhan,SUN Hui,WANG Xin,XIANG Qi-wang,ZHAO Qin,JIANG Shi-jin.Cloning and sequence analysis of ORF3 gene of duck circovirus[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(9). |
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| Authors: | WEI Zong ZOU Jin-feng LEI Zhan SUN Hui WANG Xin XIANG Qi-wang ZHAO Qin JIANG Shi-jin |
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| Institution: | WEI Zong,ZOU Jin-feng,LEI Zhan,SUN Hui,WANG Xin,XIANG Qi-wang,ZHAO Qin,JIANG Shi-jin(1.College of Veterinary Medicine,Shandong Agriculture University,Tai'an 271018,China,2.Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province,China) |
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| Abstract: | To elucidate the ORF3 gene variations of different duck circovirus(DuCV) isolates,the ORF3 gene sequences of 10 DuCV isolates,isolated from the clinical tissue samples,were analyzed and compared with that of reference DuCV strains.Sequencing results indicated that the ORF3 gene of the isolates had 2 genotypes which were 237 nt and 297 nt,respectively.The nucleotide homology of ORF3 gene in the same genotype was between 95.8% to 100%,but it was only 87.8% to 91.6% between the different genotypes.Furthermore,... |
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| Keywords: | duck circovirus ORF3 gene sequence analysis genotype |
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