| 人源H3N2亚型猪流感重组病毒的分离鉴定及全基因序列分析 |
| |
| 引用本文： | 周伦江,王隆柏,俞玉鸿,魏宏,车勇良,陈如敬,吴学敏,邵良平,庄向生.人源H3N2亚型猪流感重组病毒的分离鉴定及全基因序列分析[J].中国预防兽医学报,2011,33(9). |
| |
| 作者姓名： | 周伦江 王隆柏 俞玉鸿 魏宏 车勇良 陈如敬 吴学敏 邵良平 庄向生 |
| |
| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所/福建省畜禽疫病防治T程技术研究中心,福建福州,350013
2. 福建农林大学动物科学学院,福建福州,350002 |
| |
| 基金项目： | 国家公益性行业(农业)科研专项(NYHYZX07-034); 福建省公益类科研专项(2009K10025-5) |
| |
| 摘 要： | 本研究由疑似猪流感病料样品中分离一株病毒,通过HA、HI、电镜观察、生物学特性测定及全基因序列测定表明,分离病毒株为H3N2亚型人流感病毒和H1N1亚型古典猪流感病毒(SIV)的重组体,命名为A/Swine/Fujian/F2/07(H3N2).该分离株含有8个基因片段,共13 442 bp,与GenBank中登录的H3N2亚型人流感病毒、H1N1亚型古典SIV和H3N2亚型SIV进行比较分析显示:分离株的HA、NS、NA基因与H3N2亚型人流感病毒株的同源性分别为84.7 ％～98.1％、94.4 ％～99.5％和88.6 ％～97.6％；与H3N2亚型SIV的核苷酸同源性分别为87.7％～98.5％、82.5 ％～99.9％和87.6 ％～98.4％；M基因与H3N2亚型人流感病毒和SIV的同源性均在90.1％以下,而与H1N1亚型古典SIV的同源性在97.6％以上.基因型分析表明分离株的PB2、PBI、PA、HA、NP、NA和NS基因片段来源于1975年～1982年的人流感病毒,而M基因来源于H1N1亚型古典SIV,充分证明猪作为流感病毒“混合器”的作用.
|
| 关 键 词： | 两源重组H3N2亚型 分离 序列分析 |
Isolation and genomic sequence analysis of a human-swine H3N2 influenza reassortant viruses |
| |
| ZHOU Lun-jiang,WANG Long-bai,YU Yu-hong,WEI Hong,CHE Yong-liang,CHEN Ru-jing,WU Xue-min,SHAO Liang-ping,ZHUANG Xiang-sheng.Isolation and genomic sequence analysis of a human-swine H3N2 influenza reassortant viruses[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(9). |
| |
| Authors: | ZHOU Lun-jiang WANG Long-bai YU Yu-hong WEI Hong CHE Yong-liang CHEN Ru-jing WU Xue-min SHAO Liang-ping ZHUANG Xiang-sheng |
| |
| Institution: | ZHOU Lun-jiang1,WANG Long-bai1,YU Yu-hong2,WEI Hong1,CHE Yong-liang1,CHEN Ru-jing1,WU Xue-min1,SHAO Liang-ping2,ZHUANG Xiang-sheng1(1.Fujian Animal Disease Control Technology Development Center,Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China,2.College of Animal Science,Fujian Agricultural and Forestry University,Fuzhou 350002,China) |
| |
| Abstract: | A virus was isolated from the pig tissue sample of swine influenza-like case and identified as a H3N2 swine influenza virus(SIV) by HA and HI test,designated A/Swine/Fujian/F2/07(H3N2).The virus titers were 10-4.8 ELD50 and 10-6.17 EID50 in inoculated chicken embryos.The results of sequence analysis indicated that the virus nucleotide sequences shared 84.7% to 98.1%,94.4% to 99.5%,88.6% to 97.6% with HA,NS,NA gene of human influenza virus(HIV) reference strains,respectively,and 87.7% to 98.5%,82.5% to 99.9%... |
| |
| Keywords: | human-swine H3N2 subtype isolation gene sequencing |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
