| 致鹅卵黄性腹膜炎E.coli fimFGH基因序列分析 |
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| 引用本文： | 钱琨,张永志,金文杰,秦爱建.致鹅卵黄性腹膜炎E.coli fimFGH基因序列分析[J].中国预防兽医学报,2011,33(11):854-857. |
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| 作者姓名： | 钱琨 张永志 金文杰 秦爱建 |
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| 作者单位： | 扬州大学禽类预防医学省部共建教育部重点实验室 江苏省动物预防医学重点实验室,江苏扬州,225009 |
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| 基金项目： | 江苏省高校自然科学重大基础研究项目 |
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| 摘 要： | 为研究致鹅卵黄性腹膜炎大肠杆菌(E.coli) fimFGH基因的变异情况,本实验根据GenBank中登录的序列设计引物,PCR扩增了3株致鹩卵黄性腹膜炎性E.coli E0238、E0239、E0240 Ⅰ型菌毛的fimFGH基因并测序.序列比对分析结果表明,3株细菌的fimF、fimG和fimH基因及其所编码的蛋白...
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| 关 键 词： | 鹅卵黄性腹膜炎 E.coli fimF fimG fimH 序列分析 |
FimFGH gene sequence analysis of the Escherichia coli isolates from geese associated with yolk-sac peritonitis |
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| QIAN Kun,ZHANG Yong-zhi,JIN Wen-jie,QIN Ai-jian.FimFGH gene sequence analysis of the Escherichia coli isolates from geese associated with yolk-sac peritonitis[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(11):854-857. |
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| Authors: | QIAN Kun ZHANG Yong-zhi JIN Wen-jie QIN Ai-jian |
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| Institution: | QIAN Kun,ZHANG Yong-zhi,JIN Wen-jie,QIN Ai-jian(Ministry of Education Key Laboratory for Avian Preventive Medicine,Key Laboratory of Jiangsu Preventive Veterinary Medicine,College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China) |
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| Abstract: | To analysis the fimFGH genes of Escherichia coli isolated from geese associated with yolk-sac peritonitis,a pair of the specific primers were designed and synthesized according to the fimFGH gene in GenBank.The fimFGH genes in E.coli isolates of E0238,E0239 and E0240 were amplified and sequenced.The sequence analysis demonstrated that fimF,fimG and fimH genes were over 96% homology among the 3 E.coli isolates.Of which the deduced amino acid sequence of fimH gene was up to 98% identity and 4 major mutations ... |
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| Keywords: | yolk-sac peritonitis of geese E coli fimF fimG fimH sequence analysis |
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