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| 三株芽孢杆菌对沙门氏杆菌的体外拮抗作用研究 | |
| 引用本文： | 颜其贵,王新,郭万柱,袁孟伟,宋振辉,殷华平,李璟,曹洪志,陈斌.三株芽孢杆菌对沙门氏杆菌的体外拮抗作用研究[J].中国预防兽医学报,2007,29(1):71-74. |
| 作者姓名： | 颜其贵 王新 郭万柱 袁孟伟 宋振辉 殷华平 李璟 曹洪志 陈斌 |
| 作者单位： | 1. 四川农业大学,动物生物技术中心,四川,雅安,625014 2. 成都市动物防疫监督总站,四川,成都,610041 3. 成都理工大学,四川,成都,610061 4. 四川省动物防疫监督总站,四川,成都,610041 |
| 摘 要： | 本实验以三株芽孢杆菌为材料，采用与沙门氏菌同时接种及先后接种的方法，对其与沙门氏菌的生物拮抗作用进行了研究。结果表明：X1、X2、X3三株芽孢杆菌与沙门氏菌接种后共培养时对沙门氏菌均呈现明显的抑制作用．12h后开始呈现一定的抑制作用，24h时作用最明显，至48h时也还有一定的抑制作用，其抑菌作用依次为：12h时XI〉X2〉X3，24h时X2〉X3〉X1，48h时X1〉X2，X3对沙门氏杆菌没有抑制作用。接种X1、X2、X3三株芽孢杆菌培养24h后接种沙门氏菌对沙门氏菌均呈现明显的抑制作用，8h后开始呈现一定的抑制作用，12h左右作用最明显，24h后效果却不太明显，其抑菌作用依次为：12h时X1〉X3〉X2。接种沙门氏菌培养12h后接种X1、X2、X3三株芽孢杆菌对沙门氏菌也呈现明显的抑制作用，6h后开始呈现一定的抑制作用，8h时作用最明显，至24h时也还有一定的抑制作用，其抑菌作用依次为：8h时X2〉X1〉X3。与对照组比较，统计分析表明：（1）同时接种共培养条件下，接种X1后6h～72h内差异极显著（P〈0．01）；接种X2后6h～24h和48h～72h内差异极显著（P〈0．01），24h～48h差异显著（P〈0．05）；接种X3后0h～72h内差异不显著（P〉0．05）。（2）接种X1、X2、X3培养后接种沙门氏菌条件下，接种X1后0h～72h内差异极显著（P〈0．01）；接种X2后0h～6h和24h～48h内差异显著（P〈0．05），6h～24h为差异极显著（P〈0．01）；接种X3后0h～72h内差异不显著（P〉0．05）。（3）接种沙门氏菌后接种X1、X2和X3条件下，接种X1后6h～8h和12h～36h内差异极显著（P〈0．01），8h～12h为差异显著（P〈0．05）；接种X2后6h～8h和12h～36h内差异极显著（P〈0.01）；接种X3后24h～36h内差异显著（P〈0．05）。 |
| 关 键 词： | 芽孢杆菌 沙门氏杆菌 拮抗作用 |
| 文章编号： | 1008-0589（2007）01-0071-04 |
| 收稿时间： | 2005-10-13 |
| 修稿时间： | 2005年10月13 |
Effect of bacillus strain X1, X2 and X3 inhibiting the growth of enteric Salmonella in vitro |
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| YAN Qi-gui,WANG Xin,GUO Wan-zhu,YUAN Meng-wei,SONG Zhen-hui,YIN Hua-ping,LI Jing,CAO Hong-zhi,CHEN Bin.Effect of bacillus strain X1, X2 and X3 inhibiting the growth of enteric Salmonella in vitro[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(1):71-74. | |
| Authors: | YAN Qi-gui WANG Xin GUO Wan-zhu YUAN Meng-wei SONG Zhen-hui YIN Hua-ping LI Jing CAO Hong-zhi CHEN Bin |
| Abstract: | The effects of 3 Bacillus strains, X1, X2 and X3 inhibiting the growth of enteric Salmonella were investigated by the co-incubation, pre-incubation and post-incubation method. The result revealed that the 3 Bacillus strains showed different ability to inhibit the growth of Salmonella after co-incubated with X1, X2 and X3 respectively. Powerful inhibiting ability to Salmonella occurred at 12 h-24 h in the means of co-incubation both of simultaneously and successively, and at 8 h-12 h in the means of incubation Salmonella first and then co-incubation X1, X2 and X3. Statistical data showed that by co-incubation, pre-incubation and post-incubation X1 with Enteric Salmonella, the inhibiting effects were most obviously difference(P<0.01)at 0 h-72 h, 0 h-72 h, and both of 6 h-8 h and 12 h-36 h, respectively; and were obviously difference (P<0.05) at 8 h-12 h by post-incubation X1 with enteric Salmonella. The inhibiting effects were most obviously difference (P<0.01) at both of 0 h-24 h and 48 h-72 h, 6 h-24 h, and both of 6 h-8 h and 12 h-36 h. By co-incubation, pre-incubation and post-incubation X2 with enteric Salmonella; and were obviously difference (P<0.05) at 24 h-48 h, both of 0 h-6 h and 24 h-48 h by co-incubation and pre-incubation X2 with enteric Salmonella. By co-incubation, pre-incubation and post-incubation X3 with enteric Salmonella, the inhibiting effects were obviously difference (P<0.05) at 24 h-36 h just in post-incubation group only, no obviously differences were observed in the other two groups. |
| Keywords: | bacillus strains enteric Salmonella suis inhibition |
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