| 应用多重PCR检测人工感染鸡呼吸道疾病的研究 |
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| 引用本文： | 庞耀珊,谢芝勋,谢志勤,刘加波,邓显文,廖敏,M.I.Khan.应用多重PCR检测人工感染鸡呼吸道疾病的研究[J].中国预防兽医学报,2001,23(6):415-418. |
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| 作者姓名： | 庞耀珊 谢芝勋 谢志勤 刘加波 邓显文 廖敏 M.I.Khan |
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| 作者单位： | 1. 广西兽医研究所,
2. 美国康涅狄格州大学兽医系禽病分子生物学研究室 |
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| 基金项目： | 该研究在美国康涅狄格州大学完成.该项目获"广西十百千人才"专项基金资助.(项目编号为99202) |
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| 摘 要： | 本文报道了IBV，NDV，ILTV，MG人工感染4周龄SPF鸡和非免疫鸡后，用多重PCR检测试验鸡的咽喉棉拭子和器官组织样品，并与传统的病原分离鉴定，血清学方法进行比较，多重PCR无论是对单一感染病原，还是对两种以上混合感染病原，其敏感性和检测速度都优于传统的鉴别诊断方法，具有较高的实用价值，可以直接应用于临床检测，服务于生产。
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| 关 键 词： | 呼吸道疾病 临床样品 多重PCR 检测 |
| 文章编号： | 1008-0589(2001)06-0415-04 |
| 修稿时间： | 2001年2月27日 |
Detection of Avian Respiratory Diseases from Experimental Infected Chickens by
Multiplex PCR |
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| PANG Yao_shan,XIE Zhi_xun,M.I.Khan,XIE Zhi_qin,LIU Jia_bo,DENG Xian_wen,LIAO Ming.Detection of Avian Respiratory Diseases from Experimental Infected Chickens by
Multiplex PCR[J].Chinese Journal of Preventive Veterinary Medicine,2001,23(6):415-418. |
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| Authors: | PANG Yao_shan XIE Zhi_xun MIKhan XIE Zhi_qin LIU Jia_bo DENG Xian_wen LIAO Ming |
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| Abstract: | IBV,ILTV,NDV and MG are four major avian respiratory pathogens in poultry industry.For rapid diagnosis,a multiplex PCR was preliminary developed.In order to evaluate the efficacy of the multiplex PCR to detect and differetiate more than one respiratory agent from practic clinical specimen.4_week_old SPF chickens and nonvaccination chickens were experimentally inoculated with the four pathogens in different ways.Clinical specimen collected from inoculated and contact_infected chickens were simutaniously subjected to the multiplex PCR and traditional methods such as,pathogen isolation and sereological test.The analysis showed that the multiplex PCR was much more rapid and sensitive than the traditional methods.The results suggested that the multiplex PCR was a useful and applicable tecnique to the poultry industry. |
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| Keywords: | Avian Respiratory pathogens Clinical specimen Multiplex polymeras Chain Reaction |
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