| 植物乳杆菌BLPS-9中试发酵工艺优化及冻干保护剂筛选 |
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| 引用本文： | 辛国芹,汪祥燕,孟凡进,宁慧宇,徐海燕,谷巍.植物乳杆菌BLPS-9中试发酵工艺优化及冻干保护剂筛选[J].中国饲料,2021(1):23-27. |
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| 作者姓名： | 辛国芹 汪祥燕 孟凡进 宁慧宇 徐海燕 谷巍 |
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| 作者单位： | 山东省动物微生态制剂重点实验室;寿光市文家街道畜牧兽医工作站 |
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| 基金项目： | 国家重点研发计划项目(2017YFD0501006)。 |
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| 摘 要： | 本研究以植物乳杆菌BLPS-9为研究对象,对其中试发酵工艺进行优化,采用正交因素对其保护剂配方进行筛选。结果表明:经优化后植物乳杆菌BLPS-9中试发酵液活菌数可达50×10^8 cfu/mL,确定最佳离心条件10000 r/min、离心10 min活菌数最高,保护剂配方为脱脂奶粉25%、海藻糖7.5%、甘油0.75%;在最适离心条件下采用最佳冻干保护剂进行冷冻干燥,植物乳杆菌BLPS-9存活率可达91.01%,菌粉活菌数为6.0×10^11 cfu/g。在4℃和-20℃条件下贮存28 d,活菌数无明显损失。本研究为进一步提高乳酸菌制品的货架期提供了参考。
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| 关 键 词： | 植物乳杆菌 发酵工艺 优化 冻干保护剂 |
Optimization of pilot fermentation process of Lactobacillus plantarum BLPS-9 and screening of freeze-drying protective agent |
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| XIN Guoqin,WANG Xiangyan,MENG Fanjin,NING Huiyu,XU Haiyan,GU Wei.Optimization of pilot fermentation process of Lactobacillus plantarum BLPS-9 and screening of freeze-drying protective agent[J].China Feed,2021(1):23-27. |
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| Authors: | XIN Guoqin WANG Xiangyan MENG Fanjin NING Huiyu XU Haiyan GU Wei |
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| Institution: | (Shandong Key Laboratory of Animal Micro-ecological Agent,Shandong Baolai-leelai Bioengineering Co.,Ltd.,Tai’an,Shandong Province 271000,China;Animal Husbandry and Veterinary Workstation,Wenjia Street,Shouguang City,Shouguang,Shandong Province 262712,China) |
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| Abstract: | Taking Lactobacillus plantarum BLPS-9 as the research object,the pilot fermentation procedure was optimized,and the formulation of its protectant was screened by orthogonal factors.The results showed that the number of viable bacteria in the medium-sized fermentation broth of Lactobacillus plantarum BLPS-9 could reach 50×10^8 cfu/mL after optimization.The optimal centrifugation conditions were 10000 r/min and 10 min.The formula of survival rate of Lactobacillus plantarum BLPS-9 could reach 91.01%and the viable bacteria powder could be freeze dried under the optimal centrifugation conditions.The number was 6.0×10^11 cfu/g,and there was no significant loss of viable bacteria after 28 days storage at 4℃and-20℃.It provides the basis for further improving the shelf life of Lactobacillus products. |
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| Keywords: | Lactobacillus plantarum fermentation process optimization freeze-protecting agent |
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