| 3个绵羊群体MSTN基因编码区PCR-SSCP分析 |
| |
| 引用本文： | 任有蛇,;白元生,;杨子森,;师周戈,;刘晓妮,;张春香.3个绵羊群体MSTN基因编码区PCR-SSCP分析[J].中国养羊,2014(3):5-8. |
| |
| 作者姓名： | 任有蛇 ;白元生 ;杨子森 ;师周戈 ;刘晓妮 ;张春香 |
| |
| 作者单位： | [1]山西农业大学动物科技学院,太谷030801; [2]山西省农业厅生态畜牧产业管理站,太谷030801; |
| |
| 基金项目： | 山西省肉羊联合育种科技合作项目 |
| |
| 摘 要： | 采用PCR-SSCP技术分析了3个不同绵羊品种（杜泊绵羊、小尾寒羊、晋中绵羊）肌肉生长抑制素（MSTN）基因的单核苷酸多态性.根据3个绵羊品种MSTN基因Exon1（P1）、Exon2（P2和P3）和Exon3（P4和P5）序列设计5对PCR-SSCP引物并扩增,经SSCP分析,P1位点检测到A和B两个等位基因,AA、AB、BB三种基因型;且在晋中绵羊上处于Hardy-Weinberg平衡状态,而在小尾寒羊和杜泊绵羊上极显著偏离Hardy-Weinberg平衡状态.P2和P5位点没有检测到多态位点.P3位点检测到A和B两个等位基因,AA、BB两种基因型;且在3个绵羊品种上极显著偏离Hardy-Weinberg平衡状态.P4位点检测到A、B和C三个等位基因,AA、AB、AC三种基因型;且在小尾寒羊上处于Hardy-Weinberg平衡状态,在晋中绵羊上处于Hardy-Weinberg不平衡状态,在杜泊绵羊上极显著偏离Hardy-Weinberg平衡状态.
|
| 关 键 词： | MSTN PCR-SSCP 等位基因 多态性 |
PCR-SSCP Analysis on MSTN Gene in the Coding Region of Three Sheep Breeds |
| |
| Institution: | Ren You-she, Bai Yuan-sheng, Zhang Chun-xiang, et al ( 1.College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China; 2.Shanxi Bureau of Animal Husbandry and Veterinary) |
| |
| Abstract: | The single nucleotide polymorphisms of myostatin （MSTN）gene in the three sheep breeds （Dorper sheep,Small-tailed Han Sheep and Jinzhong sheep）were analysed in this paper.According to the sequences of Exon1 （P1）,Exon2（P2 and P3）,Exon3 （P4 and P5）ofMSTN gene in the sheeps,5 pairs of PCR-SSCP primers were designed and amplified by PCR-SSCP,The results showed that there were 2 alleles and AA,AB and BB genotypes in P1.the P1 loci in Jinzhong sheep were consistent with the Hardy-Weinberg equilibrium and in the Small Tailed Han sheep and Dorper sheep were significantly deviated from it （P ＜ 0.01）.Polymorphism was not detected in P2 and P5.There were 2 alleles and AA and AB genotypes in P3,and three sheep breeds were significantly deviated from the Hardy-Weinberg equilibrium （P ＜ 0.01）.There were 3 alleles and AA,AB,and AC genotypes in P4.the P4 loci in the Small Tailed Han sheep were consistent with the Hardy-Weinberg equilibrium,in Jinzhong sheep not,and in Dorper sheep significantly deviated from it （P ＜ 0.01）. |
| |
| Keywords: | MSTN PCR-SSCP allele polymorphism |
| 本文献已被 维普 等数据库收录！ |
|
