Genetic diversity of benign Theileria parasites of cattle in the Okinawa Prefecture |
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| Authors: | Zakimi Satoshi Kim Jung-Yeon Oshiro Mamoru Hayashida Kyoko Fujisaki Kozo Sugimoto Chihiro |
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| Institution: | Okinawa Prefectural Institute of Animal Health, Naha, Okinawa, Japan. |
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| Abstract: | Benign Theileria parasites of cattle distributed in the Okinawa prefecture were characterized by allele-specific polymerase chain reaction (PCR) and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. Using universal or allele-specific primer sets, parasite DNA was amplified in 31 out of 48 blood samples obtained from beef cattle. Among the positive cases, mixed infections involving various combinations of I-, C-, and B-type parasites were detected in 24 (77.4%) samples. Phylogenetic analysis based on the MPSP gene sequences revealed that parasites with the MPSP types 1-5 and 7, exist within the Okinawa prefecture. |
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