Epidemiological survey of Theileria parasite infection of cattle in Northeast China by allele-specific PCR |
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| Authors: | Yu Longzheng Zhang Shoufa Liang Wanfeng Jin Chunmei Jia Lijun Luo Yuzi Li Yan Cao Shinuo Yamagishi Junya Nishikawa Yoshifumi Kawano Suguru Fujisaki Kozo Xuan Xuenan |
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| Institution: | National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan. |
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| Abstract: | An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China. |
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