| 2012年-2015年我国部分地区禽偏肺病毒的分子流行病学分析 |
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| 引用本文： | 朱艳梅,宫晓,郭伟伟,徐保娟,李陆梅,郎枫,刘红祥,刘东,范根成.2012年-2015年我国部分地区禽偏肺病毒的分子流行病学分析[J].动物医学进展,2016(10). |
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| 作者姓名： | 朱艳梅 宫晓 郭伟伟 徐保娟 李陆梅 郎枫 刘红祥 刘东 范根成 |
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| 作者单位： | 动物基因工程疫苗国家重点实验室/青岛易邦生物工程有限公司,山东青岛,266114 |
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| 摘 要： | 为了调查鸡群中禽偏肺病毒（aMPV）的流行情况，本研究从江苏、辽宁、河南、山东、河北等地有肿头症状的鸡群中取其鼻甲骨和鼻黏液进行 RT-PCR 检测，随机选取9株 PCR 阳性产物对其 F 基因进行序列测定与遗传进化分析。结果表明，280份病料中检出阳性样品142份，阳性检出率达50．7％；9个试验毒株之间 F 基因同源性为99．4％～100％，与 B 型 aMPV 代表株的同源性为97．7％～99．9％，而与 A 型、C型和 D 型 aMPV 代表株的同源性为71．9％～79．4％；由遗传进化树可见，这9株 aMPV 均属于 B 型分支。说明我国鸡群中目前存在 aMPV 感染，B 型毒株是优势流行基因型，从而为禽偏肺病毒疫苗的开发提供了流行病学资料。
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| 关 键 词： | 禽偏肺病毒 流行病学 F基因 同源性 |
Molecular Epidemiology Analysis of aMPV in Some Regions in China during 2012 to 2015 |
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| Abstract: | To investigate the molecular epidemiology of aMPV in China,turbinate and nasal mucus of hen flocks with swollen head symptoms were collected from Jiangsu,Liaoning,Henan,Shandong and Hebei and tested by RT-PCR.9 PCR positive products were randomly selected,and the sequence and genetic evolution of F gene were analyzed.The results indicated that 142 of 280 samples were positive and the positive rate was 50.7%.The F gene homology was 99.4% to 100% among 9 samples,and that the homology of the B type aMPV strains was 97.7% to 99.9%;whereas the homologies of the A type,C type and D type were 71.9% to 79.4%.Moreover,the analysis of phylogenetic tree showed that all 9 tested strains belong to B genotype.In conclusion,there are current aMPV infections in broiler flocks in China,and the B strain is the dominant genotype.This study provided the basis for the development of aMPV vaccine. |
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| Keywords: | Avian metapneumovirus epidemiology F gene homology |
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