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鸡源沙门菌的耐药性及脉冲场凝胶电泳分型研究
引用本文:董剑辉,熊惠军,宋立,陈瑞.鸡源沙门菌的耐药性及脉冲场凝胶电泳分型研究[J].动物医学进展,2011,32(5):9-13.
作者姓名:董剑辉  熊惠军  宋立  陈瑞
作者单位:董剑辉,DONG Jian-hui(华南农业大学兽医学院,广东,广州,510642;中国兽医药品监察所,北京,100081);熊惠军,陈瑞,XIONG Hui-jun,CHEN Rui(华南农业大学兽医学院,广东,广州,510642);宋立,SONG Li(中国兽医药品监察所,北京,100081)
基金项目:国家"十一五"科技支撑子项目,国家科技支撑计划项目
摘    要:为了解北京地区鸡源沙门菌血清型的分布、耐药性以及基因型情况,利用Riboprinter全自动微生物基因指纹鉴定仪对分离到的沙门菌进行血清型鉴定,采用微量肉汤稀释法进行药物敏感性测定,应用脉冲场凝胶电泳(PFGE)进行基因分型.结果显示,共分离到15株肠炎沙门菌、1株鼠伤寒沙门菌,沙门菌对利福平、萘啶酸和多黏菌素E的耐药...

关 键 词:沙门菌  耐药性  脉冲场凝胶电泳

Drug Resistance and Molecular Typing Study of Salmonella gallinarum by Pulsed-field Gel Electrophoresis
DONG Jian-hui,XIONG Hui-jun,SONG Li,CHEN Rui.Drug Resistance and Molecular Typing Study of Salmonella gallinarum by Pulsed-field Gel Electrophoresis[J].Progress In Veterinary Medicine,2011,32(5):9-13.
Authors:DONG Jian-hui  XIONG Hui-jun  SONG Li  CHEN Rui
Institution:DONG Jian-hui1,2,XIONG Hui-jun1,SONG Li2,CHEN Rui1(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong,510642,China,2.China Institute of Veterinary Drug Control,Beijing,100081,China)
Abstract:To investigate the serotype,drug resistance and genotype of Salmonella isolated from avian in Beijing area,the serotypes of Salmonella strains were identified by Riboprinter automatic microbiological assay and their drug sensitivity were determined by microdilution broth method,and the genotypes were determined by pulsed-field gel electrophoresis(PFGE).The result indicated that 15 S.enteritidis and 1 S.typhimurium strains were isolated.The Salmonella strains showed high rate of drug resistance to rifampin,n...
Keywords:Salmonella  drug resistance  pulsed-field gel electrophoresis  
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