| 鸭源流感病毒分离株表面蛋白基因的克隆及序列分析 |
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| 引用本文： | 唐小明,黎满香,邱伯根,范仲鑫,刘道新,邱立新,谈志详,鲁杏华,何世成.鸭源流感病毒分离株表面蛋白基因的克隆及序列分析[J].动物医学进展,2007,28(6):5-10. |
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| 作者姓名： | 唐小明 黎满香 邱伯根 范仲鑫 刘道新 邱立新 谈志详 鲁杏华 何世成 |
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| 作者单位： | 1. 湖南农业大学动物医学院,湖南长沙,410128
2. 湖南省兽医总站,湖南长沙,410007 |
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| 基金项目： | 湖南省畜牧水产科学技术研究计划项目 |
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| 摘 要： | 从湖南分离得到一株H5N1亚型禽流感病毒(AIV),首先设计合成两对HA和NA基因特异性引物,采用两步法RT-PCR,对鸭流感病毒株 A/Duck/HunanWugang/2/2004(H5N1)(简称DK/HNWG/2/04)的表面蛋白基因进行序列测定,并与国内外已经发表的H5N1亚型毒株表面蛋白基因进行序列分析和比较.结果表明,HA和NA基因全长分别约为1.7 kb和1.4 kb,分离株与14个参考毒株HA基因的同源性为95.9%～98.0%,NA基因的同源性为87.0%～98.4%.根据HA基因核苷酸序列推导HA裂解位点氨基酸,发现分离株的裂解位点包含多个碱性氨基酸,符合高致病性禽流感的特征.
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| 关 键 词： | H5N1亚型禽流感病毒 血凝素 神经氨酸酶 序列分析 同源性 |
| 文章编号： | 1007-5038（2007）06-0005-06 |
| 修稿时间： | 2007-03-21 |
The Sequence Analysis the Surface Glycoprotein Genes of a Duck Origin Influenza virus Isolate |
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| TANG Xiao-ming,LI Man-xiang,QIU Bo-gen,FAN Zhong-xin,LIU Dao-xin,QIU Li-xin,TAN Zhi-xiang,LU Xing-hua,HE Shi-cheng.The Sequence Analysis the Surface Glycoprotein Genes of a Duck Origin Influenza virus Isolate[J].Progress In Veterinary Medicine,2007,28(6):5-10. |
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| Authors: | TANG Xiao-ming LI Man-xiang QIU Bo-gen FAN Zhong-xin LIU Dao-xin QIU Li-xin TAN Zhi-xiang LU Xing-hua HE Shi-cheng |
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| Institution: | 1. College of Veterinary Medicine, Hunan Agriculture University, Changsha, Hunan, 410128, China;2. Hunan Veterinary General Station, Changsha, Hunan ,410007, China |
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| Abstract: | In this study,an avian influenza viruses(AIV)was isolated from Hunan province,and it was identified to be H5N1.According to the published sequence of H5N1 HA and NA genes,two pairs of specific primers were synthesized.The whole genetic encoding of the isolated strain of HA and NA genes were about 1.7 kb and 1.4 kb nucleotide with a single opening frame.The homology analysis showed that the homology of HA genes between the isolate and twelve reference viruses was 95.9%-98.0%,the homology of NA genes was 87.0%-98.4%.The deduced analysis of HA genes splitting site suggested that the nucleotides contain characteristic sequence of splitting site of high pathogenic AIV strain. |
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| Keywords: | Avian influenza virus H5N1 subtype hemagglutinin(HA) neuraminidase(NA) sequence analysis homology |
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