| 广西猪源H9N2亚型流感病毒的分离与鉴定 |
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| 引用本文： | 韦达有,伍和明,蒋家霞,付薇,孙翔翔,何奇松,马琳,徐贤坤.广西猪源H9N2亚型流感病毒的分离与鉴定[J].动物医学进展,2011,32(7):113-117. |
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| 作者姓名： | 韦达有 伍和明 蒋家霞 付薇 孙翔翔 何奇松 马琳 徐贤坤 |
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| 作者单位： | 1. 广西桂林市动物疫病预防控制中心,广西桂林,541002
2. 广西区动物疫病预防控制中心,广西南宁,530001 |
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| 基金项目： | 广西自然科学基金(2010GXNSFD013033) |
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| 摘 要： | 用SPF鸡胚从广西地区猪群中分离到1株H9N2型猪流感病毒(SIV),经鸡胚接种3代后出现稳定的鸡血红细胞凝集效价为27,且凝集性能被H9亚型阳性血清抑制,而不被其他亚型流感病毒阳性血清所抑制.分离株的HA基因及NA基因扩增结果显示,该毒株HA基因与流感病毒H9亚型同源性最高,NA基因与流感病毒N2亚型同源性最高,说明...
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| 关 键 词： | 猪流感病毒 H9N2亚型 分离鉴定 HA基因 NA基因 |
Isolation and Identification of a Subtype H9N2 Influenza Virus From Swine |
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| WEI Da-you,WU He-ming,JIANG Jia-xia,FU Wei,SUN Xiang-xiang,HE Qi-song,MA Lin,XU Xian-kun.Isolation and Identification of a Subtype H9N2 Influenza Virus From Swine[J].Progress In Veterinary Medicine,2011,32(7):113-117. |
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| Authors: | WEI Da-you WU He-ming JIANG Jia-xia FU Wei SUN Xiang-xiang HE Qi-song MA Lin XU Xian-kun |
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| Institution: | WEI Da-you1,WU He-ming1,JIANG Jia-xia2,FU Wei2,SUN Xiang-xiang2,HE Qi-song2,MA Lin2,XU Xian-kun2(1.Guilin Center for Animal Disease Prevention and Control,Guilin,Guangxi,541002,China,2.Guangxi Center for Animal Disease Prevention and Control,Nanning,530001,China) |
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| Abstract: | A H9N2 subtype strain of swine influenza virus(SIV) was isolated from swine in Guangxi by SPF chicken embryo.The SIV strain had a stable haemagglutiain(HA) values for 27 after there generations,and can be inhibited by H9 positive serum.The HA and NA genetic amplification showed that this strain had the height homology with the subtype H9N2 SIV,which named A/Swine/Guangxi/01/2009. |
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| Keywords: | SIV H9N2 subtype isolation and identification HA gene NA gene |
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