| NASBA技术及其在畜禽病毒检测中的应用 |
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| 引用本文： | 张荣升,鄢明华,张国伟,张莉.NASBA技术及其在畜禽病毒检测中的应用[J].动物医学进展,2011,32(7):101-104. |
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| 作者姓名： | 张荣升 鄢明华 张国伟 张莉 |
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| 作者单位： | 1. 天津农学院,天津300384;天津市畜牧兽医研究所,天津300112
2. 天津农学院,天津,300384 |
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| 基金项目： | 天津市应用基础与前沿研究计划重点项目(08JCZDJC16800) |
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| 摘 要： | NASBA即核酸序列依赖扩增技术,主要用于扩增RNA,是由一对特异性的引物介导的、连续均一的、体外核酸序列等温扩增反应过程.该技术具有操作简便,特异性强,敏感性高,快速,高通量等优点,目前已经广泛应用于人类与动物的多种病毒性疾病的检测,具有良好的应用前景.在禽流感病毒、新城疫病毒、猪传染性胃肠炎病毒、口蹄疫病毒等的检测...
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| 关 键 词： | NASBA 原理 病毒检测 |
NASBA Technology and Its Application in Detection of Livestock Viruses |
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| ZHANG Rong-sheng,YAN Ming-hua,ZHANG Guo-wei,ZHANG Li.NASBA Technology and Its Application in Detection of Livestock Viruses[J].Progress In Veterinary Medicine,2011,32(7):101-104. |
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| Authors: | ZHANG Rong-sheng YAN Ming-hua ZHANG Guo-wei ZHANG Li |
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| Institution: | ZHANG Rong-sheng1,2,YAN Ming-hua1,ZHANG Guo-wei1,ZHANG Li1(1.Tianjin Agricultural University,Tianjin,300384,China,2.Institute of Animal Science and Veterinary Medicine,300112) |
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| Abstract: | Nucleic acid sequence based amplification(NASBA) is an isothermal amplification program which is mediated by a pair of special primer in vitro.The technology could be operated easily and fast.It has been widely applied in the detection of many viruses,that infect human and animals,depended on its high specificity and sensibility.Its high specificity and sensibility has been represented in the detection of avian influenza virus,newcastle disease virus,porcine transmissible gastroenteritis virus,food and mout... |
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| Keywords: | NASBA principle viral detection |
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