| 两株猪圆环病毒2型ORF2基因的克隆和序列分析 |
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| 引用本文： | 何逸民,罗玉均,潘全会,吴翠花,曹宗喜,孔留五,李少方,张桂红.两株猪圆环病毒2型ORF2基因的克隆和序列分析[J].动物医学进展,2007,28(11):4-7. |
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| 作者姓名： | 何逸民 罗玉均 潘全会 吴翠花 曹宗喜 孔留五 李少方 张桂红 |
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| 作者单位： | 华南农业大学兽医学院,广东广州,510642 |
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| 基金项目： | 广东省自然科学基金
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农业部制标项目 |
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| 摘 要： | 根据GenBank中猪圆环病毒2型(PCV-2)ORF2基因序列,设计一对引物,从分离的PCV-2 GZ株、HN株的细胞培养物中扩增出ORF2基因(702 bp).将此基因片段克隆入pMD18-T载体,筛选获得重组质粒pMD-ORF2,并对其测序.结果表明,所克隆的ORF2基因与其他PCV-2的ORF2基因核苷酸序列同源性在90.6%～99.6%之间,推导的氨基酸序列同源性在88.9%～98.7%之间.
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| 关 键 词： | 圆环病毒2型 ORF2 序列分析 |
| 文章编号： | 1007-5038（2007）11-0004-04 |
| 修稿时间： | 2007-08-20 |
Cloning and Sequence Analysis of ORF2 Gene of Porcine circovirus Type 2 |
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| HE Yi-min,LUO Yu-jun,PAN Quan-hui,WU Cui-hua,CAO Zong-xi,KONG Liu-wu,LI Shao-fang,ZHANG Gui-hong.Cloning and Sequence Analysis of ORF2 Gene of Porcine circovirus Type 2[J].Progress In Veterinary Medicine,2007,28(11):4-7. |
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| Authors: | HE Yi-min LUO Yu-jun PAN Quan-hui WU Cui-hua CAO Zong-xi KONG Liu-wu LI Shao-fang ZHANG Gui-hong |
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| Institution: | College of Veterinary Medicine ,South China Agricultural University ,Guangzhou,Guangdong ,510624,China |
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| Abstract: | According to the published ORF2 genes sequence of PCV-2 in GenBank,one pair of PCR primers were designed.ORF2 genes were amplified from PCV-2 GZ strain and HN strain isolated by our laboratory.Then the PCR products were cloned into pMD18-T easy vector,and the recombinant plasmids were obtained.The sequences of the cloned PCR products were analyzed and compared with other PCV-2 isolates in GenBank.The results indicated that the homology of their nucleotide sequence was 90.6% to 99.6%,and it shared 90.6% to 99.6% of amino acid homology with other PCV-2 strains. |
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| Keywords: | Porcine circovirus type 2 ORF2 gene sequence analysis |
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