| 猪脑心肌炎病毒GXLC株VP1基因的原核表达及鉴定 |
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| 引用本文： | 李向涛,施开创,陈宏备,郑敏,钟诚,李军.猪脑心肌炎病毒GXLC株VP1基因的原核表达及鉴定[J].动物医学进展,2011,32(2):6-9. |
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| 作者姓名： | 李向涛 施开创 陈宏备 郑敏 钟诚 李军 |
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| 作者单位： | 广西大学动物科学技术学院;广西动物疫病预防控制中心; |
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| 基金项目： | 广西科学基金项目(桂科青0728047); 广西科技创新能力与条件建设基金项目(08-05-01D) |
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| 摘 要： | 根据猪脑心肌炎病毒(EMCV)GXLC株的基因组序列设计一对特异性引物,应用RT-PCR方法扩增EMCV VPl基因目的片段,将其克隆至原核表达载体pET-32a(+),构建了EMCV VPl基因重组表达质粒pET-32a-VP1.将pET-32a-VP1转化BL21(DE3)株感受态细胞,并用IPTG进行诱导表达.结...
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| 关 键 词： | 脑心肌炎病毒 VP1基因 原核表达 抗原性 猪 |
Prokaryotic Expression and Identification of VP1 Gene of Encephalomyocarditis Virus GXLC Strain from Swine |
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| LI Xiang-tao,SHI Kai-chuang,CHEN Hong-bei,ZHENG Min,ZHONG Cheng,LI Jun.Prokaryotic Expression and Identification of VP1 Gene of Encephalomyocarditis Virus GXLC Strain from Swine[J].Progress In Veterinary Medicine,2011,32(2):6-9. |
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| Authors: | LI Xiang-tao SHI Kai-chuang CHEN Hong-bei ZHENG Min ZHONG Cheng LI Jun |
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| Institution: | LI Xiang-tao1,SHI Kai-chuang2,CHEN Hong-bei1,ZHENG Min2,ZHONG Cheng1,LI Jun2(1.College of Animal Science and Technology,Guangxi University,Nanning,Guangxi,530005,China,2.Guangxi Center for Animal Disease Control and Prevention,530001,China) |
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| Abstract: | One pair of primers were designed according to the genomic sequence of porcine encephalomyocarditis virus(EMCV)GXLC strain,and the VP1 gene was amplified by RT-PCR.The target fragment was cloned into prokaryotic expression vector pET-32a(+) to construct a recombinant expression vector pET-32a-VP1.Then,the pET-32a-VP1 plasmid was transformed into E.coli BL21(DE3)strain and induced by IPTG to produce recombinant VP1 protein.The results of SDS-PAGE analysis showed that the recombinant VP1 protein was efficient... |
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| Keywords: | Encephalomyocarditis virus VP1 gene prokaryotic expression antigenicity swine |
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