| 新城疫病毒山东分离株ShD-5-06 F和HN基因序列分析 |
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| 引用本文： | 岳楚昕,莫贞峰,李颖,刘栋,朱剑英.新城疫病毒山东分离株ShD-5-06 F和HN基因序列分析[J].动物医学进展,2008,29(8). |
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| 作者姓名： | 岳楚昕 莫贞峰 李颖 刘栋 朱剑英 |
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| 作者单位： | 1. 济南市畜牧办公室,山东济南,250002
2. 济南市畜牧办公室,山东济南,250002;山东农业大学动物科技学院,山东泰安,271018 |
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| 摘 要： | 从山东济南某非典型新城疫发病鸡群中分离到一株新城疫病毒株（ShD-5—06），研究其生物学特性表明，该病毒具有新城疫强毒株的一些特征。从该分离株扩增出其F和HN基因，并与标准株进行同源性比较，为探讨NDV是否发生变异提供理论依据。本试验通过RT—PCR法特异性地扩增出F和HN基因全基因序列，并对其与已经发表的序列进行核苷酸序列测定和分析。结果表明，ShD-5—06株的F和HN基因开放性阅读框架（ORF）为1662bp和1716bp，分别编码489个和571个氨基酸。与国外发表的部分新城疫病毒强毒株和弱毒株之间相应序列进行比较，F基因核苷酸序列的同源性在84．1％～88．7％之间，氨基酸同源性在88．1％～93．3％之间；HN基因核苷酸序列的同源性在82．19，5～87．4％之间，氨基酸同源性在88．6％～90．9%之间；F蛋白裂解位点区（112～117）氨基酸组成与强毒株一致，说明NDV山东分离株（ShD-5—06）为新城疫强毒株。
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| 关 键 词： | 新城疫病毒 生物学特性 F基因 HN基因 序列分析 |
Sequence and Analyses on the F and HN Gene of Newcastle disease virus ShD-5-06 Isolated in Shandong |
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| YUE Chu-xin,MO Zhen-feng,LI Ying,LIU Dong,ZHU Jian-ying.Sequence and Analyses on the F and HN Gene of Newcastle disease virus ShD-5-06 Isolated in Shandong[J].Progress In Veterinary Medicine,2008,29(8). |
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| Authors: | YUE Chu-xin MO Zhen-feng LI Ying LIU Dong ZHU Jian-ying |
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| Abstract: | A strain of atypica l Newcastle disease virus(NDV) was isolated from chickens without typical symptom in Jinan.The F gene and HN gene of NDV ShD-5-06 were cloned and compared with the published virulent strains and avirulent ones of NDV to indicate whether belong to new genotype.Fusion(F) gene and hemagglutinnin-neuriminidase(HN) gene of it were amplified by RT-PCR.F gene and HN gene were sequenced after the products of amplification being cloned and compared with the published virulent strains and avirulent ones of NDV.Based on the experiments,the F gene and HN gene of NDV ShD-5-06 had a 1662bp-1716bp open reading fragment(ORF) and encoded 489 and 571 amino acids.Compared with the published virulent strains and avirulent ones of NDV,the homology of the nucleotide of F gene is 84.1%-88.7% and the homology of the deduced amino acid sequences of F gene is 88.1%-93.3%;the homology of the nucleotide of HN gene is 82.1%-87.4% and the homology of the deduced amino acids sequences of HN gene is 88.6%-90.9%.The amino acid sequences of cleavage site regeion(112-117) of F gene were similar to that of all other virulent strains.The results also showed that the Shandong isolate(ShD-5-06) was a virulent strain of NDV. |
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| Keywords: | Newcastle disease virus biological characteritics F gene HN gene sequence analysis |
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