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| 猪口蹄疫O型合成肽疫苗抗体水平检测 | |
| 引用本文： | 陈方志,邱伯根,刘道新,谈志详,范仲鑫,邱立新,鲁杏华,何世成,唐小明,黄建龙,汪洪冰.猪口蹄疫O型合成肽疫苗抗体水平检测[J].动物医学进展,2009,30(8):116-118. |
| 作者姓名： | 陈方志 邱伯根 刘道新 谈志详 范仲鑫 邱立新 鲁杏华 何世成 唐小明 黄建龙 汪洪冰 |
| 作者单位： | 1. 湖南农业大学动物医学院,湖南长沙,410128;湖南省动物疫病预防控制中心,湖南长沙,410007 2. 湖南省兽医局,湖南长沙,410006 3. 湖南省动物疫病预防控制中心,湖南长沙,410007 |
| 摘 要： | 为了解猪口蹄疫O型合成肽疫苗的免疫效果,选用了两个厂家生产的猪口蹄疫O型合成肽疫苗,对80头试验仔猪进行了免疫注射,并在6、7、8、9、11、13、20周龄采血,通过ELISA检测抗体水平的变化。结果显示,猪口蹄疫O型合成肽疫苗能快速诱导产生高水平的抗体;疫苗的免疫副作用小;使用VP1ELISA和LPB-ELISA检测猪口蹄疫O型合成肽疫苗免疫抗体试验相关性较好。 |
| 关 键 词： | 猪 口蹄疫O型合成肽疫苗 抗体 酶联免疫吸附试验 |
Antibody Level Detection of Swine Foot and Mouth Disease(Type O) Synthetic Peptide Vaccines |
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| CHEN Fang-zhi,QIU Bo-gen,LIU Dao-xin,TAN Zhi-xiang,FAN Zhong-xing,QIU Li-xin,LU Xin-hua,HE Shi-cheng,TANG Xiao-ming,HUANG Jian-long,WANG Hong-bing.Antibody Level Detection of Swine Foot and Mouth Disease(Type O) Synthetic Peptide Vaccines[J].Progress In Veterinary Medicine,2009,30(8):116-118. | |
| Authors: | CHEN Fang-zhi QIU Bo-gen LIU Dao-xin TAN Zhi-xiang FAN Zhong-xing QIU Li-xin LU Xin-hua HE Shi-cheng TANG Xiao-ming HUANG Jian-long WANG Hong-bing |
| Institution: | 1.College of Veterinary Medicine;Hunan Agricultural University;Changsha;Hunan;410128;China;2.Hunan Centers for Animal Disease Control and Prevention;410007;3.Hunan Veterinary Bureau;410006;China |
| Abstract: | To analyze the effect of swine foot and mouth disease(Type O) synthetic peptide vaccine,80 4-week-old pigs were vaccinated with the vaccines which were selected from two manufacturers.When the pigs were 6,7,8,9,11,13 and 20 week old,blood were taken to analyze the levels of antibody by two kinds of ELISA(one is VP1 ELISA based Ag vp1,another is LPB-ELISA based whole virion).The results showed that the synthetic peptide vaccine can quickly induced antibody at a high level,and has less side effects.The result... |
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