| 鸭肝炎病毒GD-B株生物学特性测定及多聚蛋白基因序列分析 |
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| 引用本文： | 黄良宗,刘鳗仪,王淑敏,司兴奎,顾万军.鸭肝炎病毒GD-B株生物学特性测定及多聚蛋白基因序列分析[J].动物医学进展,2011,32(11):58-60. |
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| 作者姓名： | 黄良宗 刘鳗仪 王淑敏 司兴奎 顾万军 |
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| 作者单位： | 1. 佛山科学技术学院动物医学系,广东佛山,528231
2. 佛山市南山动物防疫站,广东佛山,528000 |
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| 摘 要： | 根据Ⅰ型鸭肝炎病毒(DHV-I)基因组序列设计并合成引物,通过RT-PCR方法分段扩增鸭肝炎病毒GD-B株多聚蛋白基因并克隆测序,构建多聚蛋白核苷酸系统进化树,并测定GD-B株部分生物学特性.结果表明,GD-B株多聚蛋白基因核苷酸序列与DHV-Ⅰ型GFS99广东株同源性最高(99.5％),属Ⅰ型鸭肝炎病毒,其EID50...
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| 关 键 词： | 鸭肝炎病毒 生物学特性 多聚蛋白基因 序列分析 |
Biological Characteristics and Sequence Analysis of Polyprotein Gene of Duck Hepatitis Virus GD-B Strain |
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| HUANG Liang-zong,LIU Man-yi,WANG Shu-min,SI Xing-Kui,GU Wang-jun.Biological Characteristics and Sequence Analysis of Polyprotein Gene of Duck Hepatitis Virus GD-B Strain[J].Progress In Veterinary Medicine,2011,32(11):58-60. |
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| Authors: | HUANG Liang-zong LIU Man-yi WANG Shu-min SI Xing-Kui GU Wang-jun |
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| Institution: | HUANG Liang-zong1,LIU Man-yi2,WANG Shu-min1,SI Xing-Kui1,GU Wang-jun1(1.Department of Animal Medicine,Foshan University,Foshan,Guangdong,528231,China;2.Epidemic Diseases Prevention and Quarantine of Nanshan,Foshan,Guangdong,528000,China) |
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| Abstract: | The polyprotein gene sequences of duck hepatitis virus type1(DHV-I)GD-B strain were amplified by RT-PCR with the primers designed according to the conserved region of DHV-1 genome.The sequences of polyprotein gene were analyzed by DNAStar software and the phylogenetic trees were constructed.The results showed that the GD-B strain had closest relationship with DHV I GFS99 strain sharing 99.5% identity.The CEID50and LD50of GD-B strain were 10-4.68/0.2 mL and 10-4.13/0.3 mL respectively.The lower immune protection for GD-B strain with DHV-Ⅰstandard positive serum in neutralization test indicated that the antigenic variation could occur in GD-B strain. |
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| Keywords: | Duck hepatitis virus biological characteristics polyprotein gene sequence analysis |
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