| 2株H3N8亚型禽流感病毒HA和NA基因遗传变异分析 |
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| 引用本文： | 屈素洁,郑敏,梁媛,莫胜兰,陆文俊,粟艳琼,胡杰,李军,刘棋.2株H3N8亚型禽流感病毒HA和NA基因遗传变异分析[J].动物医学进展,2011,32(9):41-45. |
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| 作者姓名： | 屈素洁 郑敏 梁媛 莫胜兰 陆文俊 粟艳琼 胡杰 李军 刘棋 |
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| 作者单位： | 广西动物疫病预防控制中心,广西南宁,530001 |
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| 基金项目： | 国家科技重大专项子课题(2009ZX10004-214) |
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| 摘 要： | 采用RT-PCR技术扩增了2株H3N8亚型流感毒株A/duck/Guangxi/69/2009和A/chicken/Guangxi/2117/2010的HA和NA基因,并与GenBank中收录的其他毒株序列进行比较分析和遗传进化分析.结果表明,分离株的HA与NA基因全长分别为1 733 bp、1 432 bp.A/du...
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| 关 键 词： | 禽流感病毒 HA基因 NA基因 序列分析 |
Genetic Variation Analysis of HA and NA Genes of Two Isolates of Avian Influenza Virus Subtype H3N8 |
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| QU Su-jie,ZHENG Min,LIANG Yuan,MOSheng-lan,LU Wen-jun,SU Yan-qiong,HU Jie,LI Jun,LIU Qi.Genetic Variation Analysis of HA and NA Genes of Two Isolates of Avian Influenza Virus Subtype H3N8[J].Progress In Veterinary Medicine,2011,32(9):41-45. |
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| Authors: | QU Su-jie ZHENG Min LIANG Yuan MOSheng-lan LU Wen-jun SU Yan-qiong HU Jie LI Jun LIU Qi |
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| Institution: | (Guangxi Center for Animal Disease Control and Prevention,Nanning,Guangxi,530001,China) |
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| Abstract: | The HA and NA genes of two H3N8 subtype avian influenza viruses A/duck/Guangxi/69/2009 and A/chicken/Guangxi/2117/2010 were amplified by RT-PCR and then sequenced.Nucleotide sequences of the HA and NA gene of the two isolates were 1 733 bp and 1 432 bp in length,respectively.The results of the comparative sequence analysis showed that the A/duck/Guangxi/69/2009 shared high identity with A/duck/Siberia/100/01,and A/chicken/Guangxi/2117/2010 shared high identity with A/white munia/Hong Kong/4519/00 in HA gene,A/duck/Guangxi/69/2009 shared high identity with A/duck/Eastern China/91/09,and A/chicken/Guangxi/2117/2010 shared high identity with A/aquatic bird/ Korea/KN-1/04 in NA gene.The phylogenetic analysis results showed the two H3N8 subtype avian influenza viruses were located in the same phylogenetic branch.The sequence on cleavage site of HA proteins of two H3N8 isolates were both P-E-K-Q-T-R,so these two viruses belonged to low pathogenic avian influenza viruses. |
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| Keywords: | Avian influenza virus HA gene NA gene sequence analysis |
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