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壳聚糖对5种细菌体外的抑制试验
引用本文:路振香,路颖,商常发,李贺侠,张宁.壳聚糖对5种细菌体外的抑制试验[J].动物医学进展,2006,27(3):62-64.
作者姓名:路振香  路颖  商常发  李贺侠  张宁
作者单位:1. 安徽科技学院,安徽凤阳,233100
2. 安徽理工大学,安徽淮南,232001
基金项目:安徽省教育厅自然科学基金;安徽省蚌埠市科技基金
摘    要:为开发利用壳聚糖,选择两种不同性质壳聚糖进行体外抑菌试验。将白色壳聚糖(Ⅰ组)和褐色壳聚糖(Ⅱ组)的乙酸溶液、褐色壳聚糖水溶液(Ⅲ组)用无菌蒸馏水分别稀释至10,5,2.5,1.25 g/L和0.625 g/L,用相应稀释度乙酸溶液(Ⅳ组)作对照组。对大肠埃希菌、金黄色葡萄球菌、蜡样芽孢杆菌、绿脓杆菌、鸡伤寒沙门菌进行体外抑菌试验。结果表明,在体外,壳聚糖对上述5种细菌的生长均有不同程度的抑制作用,不同性质的壳聚糖和同一性质不同浓度的壳聚糖抑菌效果不同。

关 键 词:壳聚糖  甲壳素  抑菌作用
文章编号:1007-5038(2006)03-0062-03
收稿时间:2005-11-22
修稿时间:2005年11月22

The Experiement of Bacteriostases of Chitosan in Vitro
LU Zhen-xiang,LU Ying,SHANG Chang-fa,LI He-xia,ZHANG Ning.The Experiement of Bacteriostases of Chitosan in Vitro[J].Progress In Veterinary Medicine,2006,27(3):62-64.
Authors:LU Zhen-xiang  LU Ying  SHANG Chang-fa  LI He-xia  ZHANG Ning
Institution:1. Anhui Science and Technology University, Fengyang,Anhui, 233100 ,China; 2. Anhui Science and Engineering University, Huainan ,Anhui, 232001 , China
Abstract:To study the role of bacteriostasis of chitosan and provide scientific evidence for clinical application,two different kinds of chitosans were selected for the experiment of bacteriostasis in vitro through medicine paper method.The solution of 20 g/L chitosan was prepared with acetic acid,and diluted into 10,5,2.5,1.25 g/L and 0.625 g/L,respectively.The corresponding diluted acetic acid solutions were used as control.The antibiotic experiment was done with,Staphylococcus aureus,Bacillus cereus,Escherichia coli,Pseudomonas aeruginosa and Salmonella gallinarum in vitro.The results showed that chitosan had bacteriostases to five becteria at different level.There were different bacteriostases in different kinds of chitosans at different concentrations.
Keywords:chitosan  chitin  bacteriostasis
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