| 不同直/支链淀粉比饲粮对育肥猪粪便中主要微生物数量和臭气物质排放的动态影响 |
| |
| 引用本文： | 屈圣富,李贞明,容庭,刘志昌,马现永,余苗.不同直/支链淀粉比饲粮对育肥猪粪便中主要微生物数量和臭气物质排放的动态影响[J].动物营养学报,2022,34(2):852-864. |
| |
| 作者姓名： | 屈圣富 李贞明 容庭 刘志昌 马现永 余苗 |
| |
| 作者单位： | 广东省农业科学院动物科学研究所,农业农村部华南动物营养与饲料重点实验室,畜禽育种国家重点实验室,广东省畜禽育种与营养研究重点实验室,广东省畜禽肉品质量安全控制与评定工程技术研究中心,广州 510640;广东省农业科学院动物科学研究所,农业农村部华南动物营养与饲料重点实验室,畜禽育种国家重点实验室,广东省畜禽育种与营养研究重点实验室,广东省畜禽肉品质量安全控制与评定工程技术研究中心,广州 510640;岭南现代农业科学与技术广东省实验室茂名分中心,茂名 525000 |
| |
| 基金项目： | 科技创新战略专项资金(高水平农科院建设)——杰出人才(R2020PY-JC001)和优秀博士(R2020YJ-YB2002);国家自然科学基金项目(31902200);广东省自然科学基金项目(2021A1515012120,2019B1515210032);广东省现代农业产业技术体系饲料创新团队项目(2020KJ115);广东省农业科学院农业优势产业科学团队建设项目(202118TD);省级农业科技创新推广及农业资源与生态环境保护建设项目(2021KJ266);韶关市科技计划项目(200731114530834)。 |
| |
| 摘 要： | 本试验旨在研究不同直/支链淀粉比饲粮对育肥猪粪便中主要微生物数量和臭气物质排放的动态影响。试验选取健康的"杜×长×大"三元杂交阉公猪72头,按初始体重(77.00±0.52) kg]相近的原则,随机分为木薯淀粉组、玉米淀粉组和豌豆淀粉组,每组8个重复(栏),每个重复3头猪。3组分别饲喂以木薯淀粉、玉米淀粉和豌豆淀粉作为唯一淀粉来源的饲粮,其直/支链淀粉的比值分别为0.11、0.25和0.44。试验预试期4 d,正试期40 d。在正试期的第1、10、20、30和40天采集育肥猪的新鲜粪便,用于主要微生物数量和臭气物质含量的测定。结果表明:1)与木薯淀粉组相比,第20和40天,豌豆淀粉组粪便中梭菌ⅩⅣ(Clostridium clusterⅩⅣ)数量显著增加(P<0.05);第30和40天,豌豆淀粉组粪便中双歧杆菌(Bifidobacterium)数量显著增加(P<0.05);第40天,豌豆淀粉组粪便中乳酸杆菌(Lactobacillus)和普雷沃氏菌属(Prevotella)数量显著增加(P<0.05);第20、30和40天,豌豆淀粉组粪便中大肠杆菌(Escherichia coli)数量显著减少(P<0.05)。2)与木薯淀粉组相比,第30和40天,豌豆淀粉组粪便中乳酸、乙酸和总挥发性脂肪酸含量显著提高(P<0.05);第20、30和40天,豌豆淀粉组粪便中丁酸含量显著提高(P<0.05)。3)第20、30和40天,豌豆淀粉组粪便pH显著低于木薯淀粉组(P<0.05)。4)与木薯淀粉组相比,第20、30和40天,豌豆淀粉组粪便中总挥发性生物胺和氨态氮(NH3-N)含量显著降低(P<0.05);第30天,豌豆淀粉组粪便中色胺和尸胺含量显著降低(P<0.05);第20和40天,豌豆淀粉组粪便中腐胺含量显著降低(P<0.05)。5)与木薯淀粉组相比,第10、20、30和40天,豌豆淀粉组粪便中吲哚含量显著降低(P<0.05);第40天,豌豆淀粉组粪便中粪臭素含量显著降低(P<0.05)。综上所述,饲喂含高直/支链淀粉比的豌豆淀粉饲粮可以增加育肥猪粪便中碳水化合物降解菌的数量,同时减少粪便中蛋白质降解菌的数量,从而降低粪便pH,改变粪便中微生物的发酵模式,最终减少粪便中NH;-N、生物胺、吲哚和粪臭素的排放,且在本试验的第20天即可产生上述效果。
|
| 关 键 词： | 淀粉 育肥猪 粪便 微生物 臭气物质 |
Dynamic Effects of Diets with Different Amylose to Amylopectin Ratios on Major Microorganism Number and Odorous Substance Mission in Feces of Finishing Pigs |
| |
| QU Shengfu,LI Zhenming,RONG Ting,LIU Zhichang,MA Xianyong,YU Miao.Dynamic Effects of Diets with Different Amylose to Amylopectin Ratios on Major Microorganism Number and Odorous Substance Mission in Feces of Finishing Pigs[J].Acta Zoonutrimenta Sinica,2022,34(2):852-864. |
| |
| Authors: | QU Shengfu LI Zhenming RONG Ting LIU Zhichang MA Xianyong YU Miao |
| |
| Institution: | (Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation,Guangdong Public Laboratory of Animal Breeding and Nutrition,State Key Laboratory of Livestock and Poultry Breeding,Key Laboratory of Animal Nutrition and Feed Science in South China,Ministry of Agriculture and Rural Affairs,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;Maoming Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Maoming 525000,China) |
| |
| Abstract: | This study aimed to investigate the dynamic effects of diets with different amylose to amylopectin ratios on major microorganism number and odorous substance mission in feces of finishing pigs. Seventy-two healthy Duroc×Landrace ×Large White crossbred barrows were randomly divided into tapioca starch group,corn starch group and pea starch group with 8 replicates(pens)per group and 3 pigs per replicate according to the initial body weight of(77.00 ±0.52)kg. The pigs in the three groups were fed diets containing tapioca starch,corn starch and pea starch as the only starch source,and the ratios of amylose to amylopectin in the diets were 0.11,0.25 and 0.44,respectively. The pre-trial period lasted for 4 days,and the formal trial period lasted for 40 days. The fresh feces of finishing pigs were collected on days 1,10,20,30 and 40 of the formal experiment,and the major microorganism number and odorous substance content in feces were analyzed. The results showed as follows:1)compared with tapioca starch group,on days 20 and 40,the Clostridium clusterⅩⅣnumber in feces in pea starch group was significantly increased(P < 0. 05);on days 30 and 40,the Bifidobacterium number in feces in pea starch group was significantly increased(P<0.05);on day 40,the numbers of Lactobacillus and Prevotella in feces in pea starch group were significantly increased(P<0.05);on days 20,30,and 40,the Escherichia coli number in feces in pea starch group was significantly decreased(P<0.05). 2)Compared with tapioca starch group,on days 30 and 40,the contents of lactic acid,acetic acid and total volatile fatty acids in feces in pea starch group were significantly increased(P<0.05);on days 20,30 and 40,the butyric acid content in feces in pea starch group was significantly increased(P<0.05). 3)On days 20,30 and 40,the pH in feces in pea starch group was significantly lower than that in tapioca starch group(P<0.05). 4)Compared with tapioca starch group,on days 20,30 and 40,the contents of total volatile biogenic amines and ammonia nitrogen(NH3-N)in feces in pea starch group were significantly decreased(P<0.05);on day 30,the contents of tryptamine and cadaverine in feces in pea starch group were significantly decreased(P<0.05);on days 20 and 40,the putrescine content in feces in pea starch group was significantly decreased(P<0.05). 5)Compared with tapioca starch group,on days 10,20,30 and 40,the indole content in feces in pea starch group was significantly decreased(P<0.05);on day 40,the skatole content in feces in pea starch group was significantly decreased(P<0.05). In conclusion,feeding pea starch diet with high amylose to amylopectin ratio can increase the number of carbon hydration degradation bacteria,and reduce the number of protein degradation bacteria in feces,thus reducing the pH and changing the fermentation pattern of microorganisms in feces,ultimately reduces some odor emission,such as NH3-N,biogenic amines,indole and skatole. And this above experiment results are realized on day 20 in this experiment.Chinese Journal of Animal Nutrition,2022,34(2):852-864] |
| |
| Keywords: | starch finishing pigs feces microbes odorous substance |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
