| 纳米氧化锌对育肥牛生产性能、血清指标和背最长肌肌内脂肪沉积相关基因表达的影响 |
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| 引用本文： | 钟奇祺,黎力之,幸清凤,郭冬生,张海波,关玮琨.纳米氧化锌对育肥牛生产性能、血清指标和背最长肌肌内脂肪沉积相关基因表达的影响[J].动物营养学报,2022,34(2):1067-1075. |
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| 作者姓名： | 钟奇祺 黎力之 幸清凤 郭冬生 张海波 关玮琨 |
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| 作者单位： | 宜春学院生命科学与资源环境学院,江西省高等学校硒农业工程技术研究中心,宜春市功能农业与生态环境重点实验室,宜春 336000;华中农业大学动物科学技术学院动物医学院,武汉 430000;宜春学院生命科学与资源环境学院,江西省高等学校硒农业工程技术研究中心,宜春市功能农业与生态环境重点实验室,宜春 336000 |
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| 基金项目： | 国家自然科学基金项目(32060773,32160804);江西省教育厅项目(GJJ190848)。 |
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| 摘 要： | 本试验旨在研究纳米氧化锌对育肥牛生产性能、血清指标及背最长肌肌内脂肪(IMF)沉积相关酶活性和基因表达的影响。选用16头西杂阉公牛(西门塔尔牛×本地黄牛),随机分2组,每组8个重复,每个重复1头牛。对照组饲喂基础饲粮(不添加锌),纳米氧化锌组在基础饲粮的基础上添加80 mg/kg锌(纳米氧化锌形式)。试验期为60 d。结果表明:1)与对照组相比,添加纳米氧化锌显著提高了育肥牛的平均日增重(P<0.05),显著降低了料重比(P<0.05)。2)与对照组相比,添加纳米氧化锌极显著提高了血清游离脂肪酸、甘油三酯、总胆固醇、高密度脂蛋白、低密度脂蛋白、葡萄糖和胰岛样生长因子-Ⅰ含量(P<0.01),显著提高了胰岛素含量(P<0.05),极显著降低了葡萄糖/胰岛素比值(P<0.01)。3)与对照组相比,添加纳米氧化锌显著提高了背最长肌IMF含量和乙酰辅酶A羧化酶(ACC)活性(P<0.05),极显著降低了激素敏感脂肪酶(HSL)活性(P<0.01),显著降低肉碱转移酶-1(CPT-1)和脂蛋白脂肪酶(LPL)活性(P<0.05)。4)与对照组相比,添加纳米氧化锌显著提高背最长肌固醇调节元件结合蛋白-1(SREBP-1)、过氧化物酶增殖激活受体γ(PPARγ)、脂肪酸合成酶(FAS)和ACC基因表达量(P<0.05),显著降低了HSL和CPT-1基因表达量(P<0.05)。由此可见,饲粮添加纳米氧化锌(80 mg/kg锌)上调育肥牛背最长肌脂肪酸合成基因(FAS、ACC、SREBP-1和PPARγ)的表达,下调脂肪酸分解基因(HSL和CPT-1)的表达,增强脂肪合成酶(FAS和ACC)的活性,抑制脂肪分解酶(HSL和CPT-1)的活性,促进IMF沉积,改善肌肉品质,提高育肥牛生产性能。
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| 关 键 词： | 育肥牛 纳米氧化锌 肌内脂肪 基因表达 |
Effects of Nano-Zinc Oxide on Performance,Serum Indexes and Expression of Intramuscular Fat Deposition Related Genes in Longissimus dorsi Muscle of Fattening Cattle |
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| ZHONG Qiqi,LI Lizhi,XING Qingfeng,GUO Dongsheng,ZHANG Haibo,GUAN Weikun.Effects of Nano-Zinc Oxide on Performance,Serum Indexes and Expression of Intramuscular Fat Deposition Related Genes in Longissimus dorsi Muscle of Fattening Cattle[J].Acta Zoonutrimenta Sinica,2022,34(2):1067-1075. |
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| Authors: | ZHONG Qiqi LI Lizhi XING Qingfeng GUO Dongsheng ZHANG Haibo GUAN Weikun |
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| Institution: | (Yichun Key Laboratory of Functional Agriculture and Ecological Environment,Engineering Technology Research Center of Jiangxi Universities and Colleges for Selenium Agriculture,College of Life Science and Resources and Environment,Yichun University,Yichun 336000,China;College of Animal Science&Technology,College of Animal Veterinary Medicine,Huazhong Agricultural University,Wuhan 430000,China) |
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| Abstract: | The purpose of this experiment was to study the effects of nano-zinc oxide on performance,serum indexes and intramuscular fat(IMF)deposition related enzyme activities and gene expression in longissimus dorsi muscle of fattening cattle. Sixteen western hybrid steers(Simmental×local yellow cattle)were randomly divided into 2 groups with 8 replicates per group and 1 cattle per replicate. In control group,cattle were fed a basal diet(without zinc),and those in experimental nano-zinc oxide group were fed the basal diet supplemented with 80 mg/kg zinc(nano-zinc oxide form). The experiment lasted for 60 days. The results showed as follows:1)compared with the control group,adding nano-zinc oxide significantly increased the average daily gain(P<0.05),and significantly decreased the feed/gain(P<0.05). 2)Compared with the control group,adding nano-zinc oxide extremely significantly increased the contents of serum free fatty acid,triglyceride,total cholesterol,high density lipoprotein,low density lipoprotein,glucose and insulin like growth factor-1(P<0.01),significantly increased insulin content(P <0.05),and extremely significantly decreased the ratio of glucose to insulin(P<0.01). 3)Compared with the control group,adding nano-zinc oxide significantly increased the IMF content and acetyl coenzyme A carboxylase(ACC)activity in longissimus dorsi muscle(P<0.05),extremely significantly decreased the hormone sensitive lipase(HSL)activity(P<0.01),and significantly decreased the activities of carnitine transferase-1(CPT-1)and lipoprotein lipase(LPL)(P<0.05). 4)Compared with the control group,adding nano-zinc oxide significantly increased the expression levels of sterol regulatory element binding protein-1(SREBP-1),peroxidase proliferation-activated receptorγ (PPARγ),fatty acid synthetase(FAS)and ACC genes in longissimus dorsi muscle(P<0.05),and significantly decreased the expression levels of HSL and CPT-1 genes in longissimus dorsi muscle(P<0.05). In conclusion,dietary nano-zinc oxide(80 mg/kg zinc)can up-regulate the expression of fatty acid synthetase related genes(FAS,ACC,SREBP-1 and PPARγ),down-regulate the expression of fatty acid decompose-related genes(HSL and CPT-1),and enhance the activities of fat synthetases(FAS and ACC)in longissimus dorsi muscle of fattening cattle,inhibit the activities of lipolytic enzyme(HSL and CPT-1),promote the deposition of IMF,improve the muscle quality and performance of fattening cattle.Chinese Journal of Animal Nutrition,2022,34(2):1067-1075] |
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| Keywords: | fattening cattle nano-zinc oxide intramuscular fat gene expression |
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