| 饲料维生素C含量对圆斑星鲽幼鱼抗氨氮胁迫能力的影响 |
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| 引用本文： | 王贞杰,叶保民,常青,陈四清,刘长琳,胡建成,严俊丽,卢斌.饲料维生素C含量对圆斑星鲽幼鱼抗氨氮胁迫能力的影响[J].动物营养学报,2016(12):334-342. |
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| 作者姓名： | 王贞杰 叶保民 常青 陈四清 刘长琳 胡建成 严俊丽 卢斌 |
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| 作者单位： | 1. 农业部海洋渔业资源可持续利用重点开放试验室，中国水产科学研究院黄海水产研究所，青岛 266071; 上海海洋大学水产与生命学院，上海 201306;2. 辽宁省海洋与渔业厅,沈阳,110000;3. 农业部海洋渔业资源可持续利用重点开放试验室，中国水产科学研究院黄海水产研究所，青岛 266071 |
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| 基金项目： | 中国水产科学研究院黄海水产研究所基本科研业务费资助(20603022016005) |
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| 摘 要： | 氨氮污染是水产养殖中重要的污染物,因此提高鱼体抗污染应激能力至关重要。本试验采用不同维生素C含量的饲料饲喂圆斑星鲽幼鱼,探究饲料维生素C含量对圆斑星鲽幼鱼抗氨氮胁迫能力的影响。试验在(12.5±1.5)℃的水温下进行,选择体重为(38.0±0.8)g的健康圆斑星鲽幼鱼,随机分为7组(每组3个重复,每个重复30尾),分别投喂维生素C含量为10.2(对照)、249.1、402.8、616.2、769.5、909.4和1 177.8 mg/kg的试验饲料8周。投喂试验结束后,从每个重复取10尾鱼,用20 mg/L的氨氮胁迫24 h。结果表明:无论是氨氮胁迫前还是氨氮胁迫后,在饲料维生素C含量达到769.5 mg/kg时,肝脏和肌肉中维生素C积累达到饱和,再继续升高饲料维生素C含量,肝脏、肌肉中维生素C含量不再显著升高(P0.05)。除1 177.8 mg/kg维生素C组外,无论是氨氮胁迫前还是氨氮胁迫后,各维生素C添加组圆斑星鲽幼鱼血清中过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性均显著高于对照组(P0.05)。除616.2 mg/kg维生素C组血清中CAT活性无显著变化(P0.05)外,氨氮胁迫使各组血清中CAT和SOD活性显著降低(P0.05),但添加维生素C可以减少降低的幅度。无论是氨氮胁迫前还是氨氮胁迫后,各维生素C添加组鳃丝Na+/K+-ATP酶活性均显著高于对照组(P0.05)。氨氮胁迫使对照组及909.4和1 177.8 mg/kg维生素C组鳃丝Na+/K+-ATP酶活性显著降低(P0.05),其他组则无显著变化(P0.05)。氨氮胁迫使各组血清中葡萄糖和乳酸含量显著升高(P0.05),对照组血清中皮质醇含量显著升高(P0.05)。此外,氨氮胁迫使各组血清中总铁结合力显著降低(P0.05)。综合各项测定指标,圆斑星鲽幼鱼饲料中维生素C含量在402.8~616.2 mg/kg时可有效提高机体抗氨氮胁迫能力。
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| 关 键 词： | 圆斑星鲽幼鱼 维生素C 应激反应 抗氨氮胁迫 |
Effects of Dietary Vitamin C Content on Anti-Ammonia-Nitrite Stress Ability of Juvenile Spotted Halibut (Verasper variegatus) |
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| Abstract: | Ammonia?nitrogen pollution is one of the important pollutants in aquaculture, thus improving the re?sistance of pollution stress of the fish is very important. This experiment using different vitamin C content diets to feed juvenile spotted halibut ( Verasper variegatus) , in order to get the effects of dietary vitamin C content on anti?ammonia?nitrogen stress ability of juvenile spotted halibut. Water temperature was maintained at (12.5± 1.5) ℃ in this experiment. Juvenile spotted halibut with the body weight of (38.0±0.8) g were randomly as?signed into 7 groups with 3 replicates per group and 30 fish per replicate, and fed 7 experimental diets with the vitamin C content was 10.2, 249.1, 402.8, 616.2, 769.5, 909.4 and 1 177.8 mg/kg, respectively. The ex?periment lasted for 8 weeks. After feeding trial, each replicate selected 10 fish to stress 24 h by 20 mg/L am?monia?nitrogen. The results showed as follows: whether before or after ammonia?nitrite stress, the vitamin C accumulation in muscle and liver reached saturation when dietary vitamin C content was 769. 5 mg/kg, and continued to rise the dietary vitamin C content, the vitamin C content in muscle and liver was not significantly increased ( P>0.05) . The activities of serum catalase ( CAT) and superoxide dismutase ( SOD) in vitamin C addition groups except 1 177. 8 mg/kg vitamin C group were significantly higher than those in control group whether before or after ammonia?nitrite stress ( P<0.05) . The activities of serum CAT and SOD in groups ex?cept serum CAT activity in 616. 2 mg/kg vitamin C group were significantly decreased by ammonia?nitrite stress ( P<0.05) , and decline ranges in vitamin C addition groups were less compared with control group. The gill Na+/K+?ATPase activity in vitamin C addition groups was significantly higher than that in control group whether before or after ammonia?nitrite stress (P<0.05). The gill Na+/K+?ATPase activity in control group and 909.4, 1 177.8 mg/kg vitamin C groups was significantly decreased ( P<0.05) , but that in other groups had no significant change after ammonia?nitrite stress ( P>0.05) . Ammonia?nitrite stress significantly increased the contents of serum glucose and lactic acid in all groups (P<0.05), and significantly increased the serum cortisol content in control group ( P<0.05) , while significantly decreased the serum total iron binding capacity (TIBC) in all groups (P<0.05). Base on a comprehensive consideration of various measured indices, when dietary vitamin C content is 402.8 to 616.2 mg/kg, the anti?ammonia?nitrogen stress ability of juvenile spotted halibut can be effectively improved. |
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| Keywords: | juvenile spotted halibut ( Verasper variegatus) vitamin C stress response anti-ammonia-nitrite stress |
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