| 慢性寒冷应激对绵羊体增重、血清免疫和抗氧化机能的影响 |
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| 引用本文： | 石璐璐,王哲奇,徐元庆,毛晨羽,郭世伟,金晓,史彬林.慢性寒冷应激对绵羊体增重、血清免疫和抗氧化机能的影响[J].动物营养学报,2021,33(1). |
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| 作者姓名： | 石璐璐 王哲奇 徐元庆 毛晨羽 郭世伟 金晓 史彬林 |
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| 作者单位： | 内蒙古农业大学动物科学学院,呼和浩特 010018;内蒙古农业大学动物科学学院,呼和浩特 010018;内蒙古农业大学动物科学学院,呼和浩特 010018;内蒙古农业大学动物科学学院,呼和浩特 010018;内蒙古农业大学动物科学学院,呼和浩特 010018;内蒙古农业大学动物科学学院,呼和浩特 010018;内蒙古农业大学动物科学学院,呼和浩特 010018 |
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| 基金项目： | 国家重点研发计划项目(2016YFD0500508)。 |
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| 摘 要： | 本试验旨在探究慢性寒冷应激对绵羊体增重、血清免疫和抗氧化机能的影响。选取体重为(55.50±0.80)kg、毛丛长度为(11.60±1.47)cm的3岁杜蒙杂交母羊18只,随机分为3组:舍内加热组(对照组)、舍内组和舍外组,每组6只。试验共28 d,每天记录各组试验羊的采食量,并于试验第1、14和28天称重,计算平均日增重(ADG)和平均日采食量(ADFI);于试验第14和28天进行颈静脉采血,分离血清并收集白细胞,测定免疫、抗氧化指标及相关基因相对表达量。结果表明:与对照组相比,1)舍外组绵羊的ADG显著降低(P<0.05);2)在试验第14天,舍外组血清白细胞介素(IL)-1含量和白细胞中IL-1β、IL-2基因相对表达量显著降低(P<0.05),舍内组血清IL-1含量和白细胞中IL-1β基因相对表达量显著降低(P<0.05);在试验第28天,舍外组血清免疫球蛋白(Ig)G、IL-2含量显著下降(P<0.05),血清肿瘤坏死因子-α(TNF-α)含量和白细胞中其基因相对表达量显著升高(P<0.05),舍内组血清IL-2含量显著下降(P<0.05);3)在试验第14天,舍外组血清谷胱甘肽过氧化物酶(GPx)、总超氧化物歧化酶(T-SOD)活性和白细胞中GPx、超氧化物歧化酶1(SOD1)基因相对表达量显著下降(P<0.05),血清丙二醛(MDA)含量显著升高(P<0.05);在试验第28天,舍外组血清过氧化氢酶(CAT)、GPx、T-SOD活性和总抗氧化能力(T-AOC)及白细胞中GPx、超氧化物歧化酶2(SOD2)基因相对表达量显著下降(P<0.05),而白细胞中CAT、核转录因子2(Nrf2)基因相对表达量和血清MDA含量显著升高(P<0.05),舍内组血清T-AOC及CAT活性和白细胞中其基因相对表达量显著下降(P<0.05)。综上所述,慢性寒冷应激能够降低绵羊的体增重,抑制机体血清免疫和抗氧化水平,而安置供暖设备的保温圈舍可以显著缓解这种负面影响。
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| 关 键 词： | 慢性寒冷应激 绵羊 免疫水平 抗氧化能力 基因表达 |
Effects of Chronic Cold Stress on Body Weight Gain,Serum Immune and Antioxidative Functions of Sheep |
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| SHI Lulu,WANG Zheqi,XU Yuanqing,MAO Chenyu,GUO Shiwei,JIN Xiao,SHI Binlin.Effects of Chronic Cold Stress on Body Weight Gain,Serum Immune and Antioxidative Functions of Sheep[J].Acta Zoonutrimenta Sinica,2021,33(1). |
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| Authors: | SHI Lulu WANG Zheqi XU Yuanqing MAO Chenyu GUO Shiwei JIN Xiao SHI Binlin |
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| Institution: | (College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China) |
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| Abstract: | This study was conducted to evaluate the effects of chronic cold stress on body weight gain,serum immune and antioxidative function of sheep.Eighteen 3-year-old Dorper×Mongolian crossbred ewes,with similar body weight of(55.50±0.80)kg and unstretched staple length of(11.60±1.47)cm,were randomly allocated into three groups:indoor heating group furnished with a radiator(control group),indoor group and outdoor group and each group had 6 sheep.The trial lasted for 28 days.Each sheep was recorded feed intake every day and body weight on days 1,14 and 28.The average daily weight gain(ADG)and average daily feed intake(ADFI)were calculated.Blood and leukocyte samples were collected on days 14 and 28 to analysis the immune and antioxidant indexes and the related gene relative expression.The results showed that,compared with control group,1)the ADG in outdoor group was significantly decreased(P<0.05);2)on day 14,the content of interleukin(IL)-1 in serum and the relative expression levels of IL-1βand IL-2 genes in leukocyte in outdoor group were significantly decreased(P<0.05).The content of IL-1 in serum and the relative expression level of IL-1βgene in leukocyte in indoor group were significantly decreased(P<0.05).On day 28,the contents of immunoglobulin(Ig)G and IL-2 in serum in outdoor group were significantly decreased(P<0.05),while the content of tumor necrosis factor-α(TNF-α)in serum and the relative expression level of its gene were significantly increased(P<0.05).The content of IL-2 in serum in indoor group was significantly decreased(P<0.05);3)on day 14,the activities of glutathione peroxidase(GPx)and total superoxide dismutase(T-SOD)in serum and the relative expression levels of GPx and superoxide dismutase 1(SOD1)genes in leukocyte in outdoor group were significantly decreased(P<0.05),while the content of malonaldehyde(MDA)in serum was significantly increased(P<0.05).On day 28,the activities of CAT,GPx and T-SOD,total antioxidant capacity(T-AOC)and the relative expression levels of GPx and SOD2 genes in leukocyte in outdoor group were significantly decreased(P<0.05),while the relative expression levels of CAT and Nrf 2 genes in leukocyte and the content of MDA in serum were significantly increased(P<0.05).The activity of CAT and T-AOC in serum and the relative expression levels of their genes in leukocyte in indoor group were significantly decreased(P<0.05).In conclusion,chronic cold stress can decrease body weight gain and suppress serum immunity and antioxidant level of sheep.However,the heat-preservation sheep house which furnished with a radiator can significantly relieve these negative effects. |
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| Keywords: | chronic cold stress sheep immune level antioxidative capacity gene expression |
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