| 枯草芽孢杆菌联合苜蓿多糖对蛋鸡生产性能、蛋品质、血液指标及粪和肠道微生物区系的影响 |
| |
| 引用本文： | 郭军蕊,董晓芳,佟建明.枯草芽孢杆菌联合苜蓿多糖对蛋鸡生产性能、蛋品质、血液指标及粪和肠道微生物区系的影响[J].动物营养学报,2017,29(5). |
| |
| 作者姓名： | 郭军蕊 董晓芳 佟建明 |
| |
| 作者单位： | 中国农业科学院北京畜牧兽医研究所,北京,100193 |
| |
| 基金项目： | "十二五"国家科技支撑计划课题，国家蛋鸡产业技术体系建设专项经费，中国农业科学院科技创新工程 |
| |
| 摘 要： | 本试验旨在研究饲粮中添加枯草芽孢杆菌及其与苜蓿多糖联用对蛋鸡生产性能、蛋品质、血液指标及粪和肠道微生物区系的影响,并观察枯草芽孢杆菌联合苜蓿多糖相较于枯草芽孢杆菌单独使用是否具有更优的效果。试验选用288只27周龄健康海兰褐蛋鸡随机分为4个组,每组6个重复,每个重复12只鸡。1组为对照组,饲喂基础饲粮;2~4组分别在基础饲粮中添加1.0×10~7CFU/g枯草芽孢杆菌、1.0×10~7CFU/g枯草芽孢杆菌+250 mg/kg苜蓿多糖、1.0×10~7CFU/g枯草芽孢杆菌+4 000 mg/kg苜蓿多糖,试验期为24周。结果表明:1)各组间采食量、蛋重、只产蛋量及死淘率无显著差异(P0.05),但与对照组相比,3、4组第9~16周的产蛋率显著提高(P0.05),2、3、4组第9~16、17~24、1~24周的料蛋比显著降低(P0.05)。2)饲粮中添加枯草芽孢杆菌及其与苜蓿多糖联合使用对蛋壳颜色、蛋壳厚度、蛋壳强度、蛋形指数、蛋白高度和哈夫单位无显著改善作用(P0.05),但显著提高第1、2、4、12周蛋黄颜色(P0.05)。3)饲粮中添加枯草芽孢杆菌及其与苜蓿多糖联合使用对第2、3、4、12、16、20周血清葡萄糖和第2、3、8、12、20周血清尿素含量有显著改善作用(P0.05),且3组第2、3、4、8周和4组第3、4、8、20周血清葡萄糖含量显著高于2组(P0.05);饲粮中添加枯草芽孢杆菌及其与苜蓿多糖联合使用显著提高血液白细胞、淋巴细胞、中间细胞和中性粒细胞数目(P0.05),3组第1、3、4、8、12周和4组第3周中间细胞数目显著高于2组(P0.05),3组第1、3、4、8周和4组第1、3、8周组中性粒细胞数目显著高于2组(P0.05);饲粮中添加枯草芽孢杆菌及其与苜蓿多糖联合使用显著提高了第3、16、24周血小板数目(P0.05)。饲粮中添加枯草芽孢杆菌及其与苜蓿多糖联合使用显著提高血清第16周免疫球蛋白A,第1和4周免疫球蛋白G和第2、4、8周免疫球蛋白M含量(P0.05),且3、4组优于试验2组(P0.05)。4)饲粮中添加枯草芽孢杆菌及其与苜蓿多糖联合使用显著降低了粪中大肠杆菌数量和盲肠大肠杆菌/乳酸菌值(P0.05),显著提高了空肠、回肠和盲肠双歧杆菌数量(P0.05)。综上,饲粮中添加枯草芽孢杆菌及其与苜蓿多糖联合使用能够改善蛋鸡生产性能和蛋品质,降低粪和盲肠大肠杆菌数量,增加肠道双歧杆菌数量,且联合使用效果在改善血清生化指标、调节机体免疫功能方面较单独使用枯草芽孢杆菌有一定优势
|
| 关 键 词： | 蛋鸡 枯草芽孢杆菌 苜蓿多糖 生产性能 蛋品质 血液指标 微生物区系 |
Effects of Bacillus subtilis and Alfalfa Polysaccharide on Performance,Egg Quality,Blood Indices and Fecal and Intestinal Microbial Flora of Laying Hens |
| |
| GUO Junrui,DONG Xiaofang,TONG Jianming.Effects of Bacillus subtilis and Alfalfa Polysaccharide on Performance,Egg Quality,Blood Indices and Fecal and Intestinal Microbial Flora of Laying Hens[J].Acta Zoonutrimenta Sinica,2017,29(5). |
| |
| Authors: | GUO Junrui DONG Xiaofang TONG Jianming |
| |
| Abstract: | The study was conducted to investigate the effects of dietary Bacillus subtilis and its combination with alfalfa polysaccharide on performance, egg quality, blood indices and fecal and intestinal microbial flora of laying hens, and to evaluate whether the combination effects were better than that of Bacillus subtilis alone or not. Two hundred and eighty?eight 27?week?old healthy Hy?Line Brown laying hens were randomly allocated into 4 groups with 6 replicates of 12 birds in each for 24 weeks. The diets were formulated by adding Bacillus subtilis and alfalfa polysaccharides to a basal diet:control group was fed with the basal diet ( group 1) , experi?mental groups were supplemented with 1.0×107 CFU/g Bacillus subtilis (group 2), 1.0×107 CFU/g Bacillus subtilis+250 mg/kg alfalfa polysaccharides (group 3) and 1.0×107 CFU/g Bacillus subtilis+4000 mg/kg al?falfa polysaccharides ( group 4 ) . The results showed as follows: 1 ) there were no significant differences in feed intake, egg weight, egg weight per hen and mortality among all groups (P>0.05). However, the egg production in groups 3 and 4 during 9 to 16 weeks was significantly increased ( P<0.05) and the ratio of feed to egg in groups 2, 3 and 4 during 9 to 16, 17 to 24 and 1 to 24 weeks was significantly decreased ( P<0.05) compared with the control group. 2) Dietary Bacillus subtilis and its combination with alfalfa polysaccharide had no significant effects on eggshell color, eggshell thickness, eggshell strength, egg shape index, albumen height and Haugh unit (P>0.05), but significantly improved egg yolk color at 1, 2, 4 and 12 weeks (P<0.05) . 3) Dietary Bacillus subtilis and its combination with alfalfa polysaccharide had significantly improved effects on serum glucose content at 2, 3, 4, 12, 16 and 20 weeks and serum urea content at 2, 3, 8, 12 and 20 weeks ( P<0.05) , and serum glucose content in group 3 at 2, 3, 4 and 8 weeks and group 4 at 3, 4, 8 and 20 weeks was increased significantly compared with group 2 ( P<0.05) . Dietary Bacillus subtilis and its combi?nation with alfalfa polysaccharide significantly increased counts of blood leucocytes, lymphocytes, intermediate cell and neutrophils ( P<0.05) , intermediate cell count in group 3 at 1, 3, 4, 8 and 12 weeks and group 4 at 3 weeks was significantly higher than that in group 2 ( P<0.05) , and neutrophils count in group 3 at 1, 3, 4 and 8 weeks and group 4 at 1, 3 and 8 weeks was significantly higher than that in group 2 ( P<0.05) . Mean?while, dietary Bacillus subtilis and its combination with alfalfa polysaccharide significantly increased platelet count at 3, 16 and 24 weeks ( P<0.05) . Dietary Bacillus subtilis and its combination with alfalfa polysaccha?ride significantly increased serum immunoglobulin A content at 16 weeks, immunoglobulin G content at 1 and 4 weeks and immunoglobulin M content at 2, 4 and 8 ( P<0.05) , and the improved effects in groups 3 and 4 were better than those in group 2 ( P<0.05) . 4) Dietary Bacillus subtilis and its combination with alfalfa poly?saccharide significantly decreased fecal Escherichia coli count and the ratio of Escherichia coli to Lactobacillus in cecum ( P<0.05) , significantly increased jejunal, ileal and cecal Bifidobacterium count ( P<0.05) . Accord?ingly, dietary Bacillus subtilis and its combination with alfalfa polysaccharide can improve performance and egg quality, reduce fecal and cecal Escherichia coli count and increase intestinal Bifidobacterium count of laying hens. Additionally, the effects of combination of Bacillus subtilis and alfalfa polysaccharide on improving ser?um biochemical indices and enhancing immunity are superior to Bacillus subtilis. |
| |
| Keywords: | laying hens Bacillus subtilis alfalfa polysaccharide performance egg quality blood indices microbial flora |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
