| 应用16S rRNA基因测序法鉴定兔支气管败血波氏杆菌的研究 |
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| 引用本文： | 李峰,管宇,沈志强,刘金华,林初文.应用16S rRNA基因测序法鉴定兔支气管败血波氏杆菌的研究[J].中国养兔杂志,2008(1):22-23,28. |
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| 作者姓名： | 李峰 管宇 沈志强 刘金华 林初文 |
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| 作者单位： | [1]山东省滨州畜牧兽医研究院,滨州256600 [2]中国农业大学动物医学院,北京100094 |
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| 摘 要： | 应用16S rRNA基因测序法对兔支气管败血波氏杆菌Bb-1株进行了16SrRNA基因序列分析。结果表明，能够扩增出与预期结果相符合的片断。经Blastn比较，分离菌与兔支气管败血波氏杆菌RB50株（BX640449）同源性为99．8％。进一步的生化实验鉴定表明，Bb-1株生化反应结果符合兔支气管败血波氏杆菌生化反应特征。综合各种实验结果表明，分离菌Bb-1株为兔支气管败血波氏杆菌。
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| 关 键 词： | 兔支气管败血波氏杆菌 16S rRNA 生化鉴定分析 |
| 文章编号： | 1006-6327（2008）01-0022-02 |
| 收稿时间： | 2007-12-05 |
| 修稿时间： | 2007年12月5日 |
Study of Bordetella Bronchiseptic Strain Identified by 16S rRNA Gene Sequencing |
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| LI Feng,GUAN Yu,SHEN Zhi-qiang,LIU Jin-hua,LIN Chu-wen.Study of Bordetella Bronchiseptic Strain Identified by 16S rRNA Gene Sequencing[J].Chinese Journal of Rabbit Farming(CJRF),2008(1):22-23,28. |
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| Authors: | LI Feng GUAN Yu SHEN Zhi-qiang LIU Jin-hua LIN Chu-wen |
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| Institution: | LI Feng,GUAN Yu,SHEN Zhi-qiang, LIU Jin-hua, LIN Chu-wen ( l.Shandong Binzhou Animal Science ＆ Veterinary Medicine Academy Binzhou 256600; 2.College of Veterinary Medicine, China Agricultual University Beijing, 100094 China ) |
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| Abstract: | One Rabbit Bordetella bronchiseptie strain Bb-1 was analysised by 16S rRNA gene sequencing. The results showed that the nucleotide sequence homology the isolated strain and the published strain RB50（BX640449） was 99.8%. The later biochemical tests indicated the isolated strain got the same biochemical event with the type strain. The results of 16S rRNA gene and biochemical tests confirmed that the isolated strain was rabbit Bordetella bronchiseptic strain. |
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| Keywords: | Rabbit Bordetella bronchiseptic stain 16S rRNA Biochemistry identification |
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