| 陕西地区羊源致病性大肠杆菌耐药性分析与毒力基因检测 |
| |
| 引用本文： | 赵学亮,王斌,苗永强,赵浩宇,谢青芳,王娟,杨增岐.陕西地区羊源致病性大肠杆菌耐药性分析与毒力基因检测[J].畜牧兽医学报,2022,53(5):1644-1648. |
| |
| 作者姓名： | 赵学亮 王斌 苗永强 赵浩宇 谢青芳 王娟 杨增岐 |
| |
| 作者单位： | 西北农林科技大学动物医学院, 杨凌 712100 |
| |
| 基金项目： | 国家绒毛用羊产业技术体系资助项目(CARS-39-14)；;陕西省自然科学基金项目(2021KW-41)； |
| |
| 摘 要： | 旨在了解陕西省部分地区腹泻羊源致病性大肠杆菌(E. coli)耐药性及毒力基因携带情况，本研究从10个养殖场采集54份腹泻羊拭子样品，经分离纯化、生化鉴定及16S rRNA基因序列分析，共分离得到50株E. coli，对分离菌进行药敏试验、耐药基因及毒力基因检测。结果显示，分离菌对氨苄西林、氟苯尼考和磺胺异噁唑耐药率达90%以上，且98%(49/50)为多重耐药菌，对8~11种抗生素耐药的菌株占68%(34/50)，仅对美罗培南敏感。所有菌株均携带1~6种不同的耐药基因，其中，Sul1(64%)、TetA(34%)、blaCTX-M(32%)携带率较高，未检测到blaSHV。有5株产ESBLs的E. coli携带mcr-1耐药基因。毒力基因检测结果显示，98%(49/50)的菌株携带毒力基因，其中，etrA检出率最高，为80%(40/50)。综上表明，陕西省羊源E. coli多重耐药情况严峻，β-内酰胺类耐药基因与耐药表型不符，提示可能存在其他耐药机制，同时，分离菌具有复杂的毒力谱。本研究为陕西省羊源致病性E. coli感染的防控提供科学依据。
|
| 关 键 词： | 大肠杆菌 致病性 耐药性 耐药基因 毒力基因 |
| 收稿时间： | 2021-08-30 |
Detection of Virulence Genes and Antimicrobial Resistance Analysis of Escherichia coli Isolated from Diarrhea Sheep in Shaanxi Province |
| |
| ZHAO Xueliang,WANG Bin,MIAO Yongqiang,ZHAO Haoyu,XIE Qingfang,WANG Juan,YANG Zengqi.Detection of Virulence Genes and Antimicrobial Resistance Analysis of Escherichia coli Isolated from Diarrhea Sheep in Shaanxi Province[J].Acta Veterinaria et Zootechnica Sinica,2022,53(5):1644-1648. |
| |
| Authors: | ZHAO Xueliang WANG Bin MIAO Yongqiang ZHAO Haoyu XIE Qingfang WANG Juan YANG Zengqi |
| |
| Institution: | College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China |
| |
| Abstract: | The aim of this study was to investigate the antimicrobial resistance and virulence genes of Escherichia coli isolated from diarrhea sheep in Shaanxi Province. In this study, 54 diarrhea swabs were collected from 10 sheep farms. After purification, biochemical identification and 16S rRNA analysis, a total of 50 E. coli strains were isolated. For E. coli isolates, antibiotic resistance, resistance genes, and virulence genes were tested. The results showed that 49 E. coli strains display multiple-drug resistance, more than 90% of the strains were resistant to ampicillin, florfenicol, and sulfisoxazole, and 100% (50/50) of the strains were sensitive to meropenem. In addition, 8-11 resistant accounts for 68% of the total isolated strains (34/50). All strains carry 1-6 different resistance genes. Among them, Sul1 (64%), TetA (34%), blaCTX-M (32%) had higher carrying rates, and blaSHV (0%) was not detected. It is noteworthy that five ESBLs-strains carry the mcr-1 gene from the isolated. The results showed that the mortality rate of mice infected with the 50 strains of E. coli was 33%-100%, virulence genes were detected in 49 of the 50 isolates, with etrA showing the highest detection rate, accounting for 80% (40/50). The results indicated that the high proportion of virulence genes and the multidrug-resistance phenomena was prevalent in sheep pathogenic E. coli. The resistance phenotypes of β-lactams were basically not correlated with the resistance genes, suggesting that there might be multiple resistance mechanisms in the E. coli strains. The virulence spectrum of isolates was complex. This study provided a scientific basis for prevention and control of the pathogenic E. coli from sheep in Shaanxi area. |
| |
| Keywords: | E coli pathogenic antibiotic resistance resistance genes virulence genes |
|
| 点击此处可从《畜牧兽医学报》浏览原始摘要信息 |
| 点击此处可从《畜牧兽医学报》下载免费的PDF全文 |
|
