| 屎肠球菌对感染柔嫩艾美耳球虫肉鸡生长性能和肠道屏障的影响 |
| |
| 引用本文： | 焦宇洲,杨欢欢,卢垚,史聪聪,徐晓娟,蔡旭旺.屎肠球菌对感染柔嫩艾美耳球虫肉鸡生长性能和肠道屏障的影响[J].畜牧兽医学报,2022,53(4):1210-1219. |
| |
| 作者姓名： | 焦宇洲 杨欢欢 卢垚 史聪聪 徐晓娟 蔡旭旺 |
| |
| 作者单位： | 1. 华中农业大学动物医学院 农业微生物学国家重点实验室, 武汉 430070;2. 武汉科缘生物发展有限责任公司, 武汉 430206 |
| |
| 基金项目： | 国家重点研发计划(2016YFD0501607)；;中央高校基本科研业务费专项基金(2020BC208)； |
| |
| 摘 要： | 旨在探究屎肠球菌(Enterococcus foecium)对人工感染柔嫩艾美耳球虫(Eimeriatenella)肉鸡生长性能、存活率、盲肠损伤、粪便卵囊排出和肠道屏障功能的影响.选取体重相近的1日龄肉鸡(罗斯308)180羽,随机分为5组,包括空白对照组(NC)、感染对照组(PC)、70 mg·kg-1盐霉素添加组...
|
| 关 键 词： | 屎肠球菌 柔嫩艾美耳球虫 肠道屏障 |
| 收稿时间： | 2021-07-02 |
Effect of Enterococcus faecium on Growth Performance and Intestinal Barrier of Broilers Infected with Eimeria tenella |
| |
| JIAO Yuzhou,YANG Huanhuan,LU Yao,SHI Congcong,XU Xiaojuan,CAI Xuwang.Effect of Enterococcus faecium on Growth Performance and Intestinal Barrier of Broilers Infected with Eimeria tenella[J].Acta Veterinaria et Zootechnica Sinica,2022,53(4):1210-1219. |
| |
| Authors: | JIAO Yuzhou YANG Huanhuan LU Yao SHI Congcong XU Xiaojuan CAI Xuwang |
| |
| Institution: | 1. State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China;2. Wuhan Keyuan Biological Development Co. Ltd., Wuhan 430206, China |
| |
| Abstract: | The present study was designed to evaluate the effect of dietary supplementation with Enterococcus faecium on growth performance, survival rate, caecal lesion, fecal oocyst excretion and intestinal barrier in broiler chickens challenged with E. tenella infection. Total of 180 one-day-old broiler chickens (Ross 308) with similar body weight were randomly distributed into 5 groups, including control group (NC), positive group (PC), salinomycin group (SAL), E. faecium group (EA and EB). Chickens in NC and PC groups were fed with basal diet, while SAL, EA, EB groups were fed with the diet supplemented with 70 mg·kg-1 salinomycin, 1.0×108 CFU·kg-1 E.faecium and 2.5×109 CFU·kg-1 E.faecium, respectively, until the end of the experiment. At 15 days of age, the chickens in all groups except NC were orally infected with 1.0×105 sporulated E. tenella oocysts. Then, the anticoccidial efficacy of dietary supplementation with E. faecium was evaluated by the average daily gain (ADG), oocyst per gram faeces (OPG), caecal lesion score, histopathological examinations and gene expression levels of cecal barrier related molecules (iNOS, MUC2, Occludin, JAM2, ZO-1). The results showed that: 1) Compared with NC group, ADG was significantly increased in EA and EB groups before treated with E. tenella (P<0.05). The ADG of SAL and EB groups were higher than that in PC group after infected with E.tenella (P<0.05). 2) Compared with PC group, the OPG in all pre-treated groups (SAL, EA, and EB) were significantly reduced (P<0.05). Besides, the obvious pathological damage with immune cell infiltration and villi blunting was observed in the cecum in PC group compared with pre-treated groups, while no significant difference was found in all pre-treated groups regard to the lesion score (P>0.05). 3) During the whole test period, the survival rates of NC, PC, SAL, EA and EB groups were 100%, 58.33%, 83.33%, 58.33% and 75.00%, respectively. 4) Compared with NC group, the expression of iNOS, MUC2, Occludin, JAM2, ZO-1 in PC group was significantly decreased (P<0.05). The expression of iNOS in all pre-treated groups and MUC2, Occludin, JAM2 in EB group showed a remarkable increase compared with PC group (P<0.05). In conclusion, the results of this study are promising and indicate many beneficial effects of E. faecium-supplementation in poultry diets to reduce the negative consequences of E. tenella disease by regulating gene expression of intestinal barrier related molecules. |
| |
| Keywords: | Enterococcus faecium Eimeria tenella intestinal barrier |
|
| 点击此处可从《畜牧兽医学报》浏览原始摘要信息 |
| 点击此处可从《畜牧兽医学报》下载免费的PDF全文 |
|
