| H9N2亚型禽流感病毒复制特性的基因分析 |
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| 引用本文： | 石火英,孙蕾,陈素娟,卢建红,刘秀梵.H9N2亚型禽流感病毒复制特性的基因分析[J].畜牧兽医学报,2008,39(2):189-194. |
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| 作者姓名： | 石火英 孙蕾 陈素娟 卢建红 刘秀梵 |
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| 作者单位： | 扬州大学兽医学院,农业部畜禽传染病学重点开放实验室,扬州,225009 |
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| 基金项目： | 江苏省属高校重大基础研究项目资助（05KJA23016）;国家自然科学基金项目资助（30670079） |
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| 摘 要： | 利用反向遗传技术，通过基因重排方法，以A/chicken/shanghai/F/98（H9N2）禽流感病毒（Avian influenza virus，AIV）的6个内部基因为骨架，与A/Chicken/Guangdong/SS/94（H9N2）AIV的HA和NA基因组合，产生3株H9N2亚型重排AIVs。动物试验发现A/Chicken/Shanghai/F/98（H9N2）和A/Chicken/Guangdong/SS/94（H9N2）AIV主要在呼吸系统复制，A/chicken/shanghai/F/98（H9N2）株在气管和肺组织的复制能力明显强于A/Chicken/Guangdong/SS/94（H9N2）AIV株。3株H9N2亚型重排AIVs的动物试验发现HA和NA基因对H9N2亚型AIV在呼吸道的复制特性起主要作用。内部基因对H9N2亚型AIV在呼吸道的复制也有一定的作用。结果表明1994年中国首次分离到的H9N2亚型AIV经过4年的宿主适应和基因进化，加强了其在呼吸系统的复制能力，奠定了气溶胶传播的基础。
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| 关 键 词： | 禽流感病毒 H9N2亚型 复制特性 反向遗传技术 HA基因 NA基因 |
| 文章编号： | 0366-6964（2008）02-0189-06 |
| 收稿时间： | 2007-04-17 |
| 修稿时间： | 2007年4月17日 |
Analysis of Genes Affecting the Replication Property of H9N2 Avian Influenza A Viruses |
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| SHI Huo-ying,SUN Lei,CHEN Su-juan,LU Jian-hong,LIU Xiu-fan.Analysis of Genes Affecting the Replication Property of H9N2 Avian Influenza A Viruses[J].Acta Veterinaria et Zootechnica Sinica,2008,39(2):189-194. |
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| Authors: | SHI Huo-ying SUN Lei CHEN Su-juan LU Jian-hong LIU Xiu-fan |
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| Abstract: | To explore the replicating feature of H9 avian influenza virus(AIV) in chickens,A/Chicken/Guangdong/SS/94(H9N2)(SS) strain,which representing the earlier strain isolated in chickens in Guangdong province in 1994, and A/Chicken/Shanghai/F/98(H9N2)(F) strain,which was isolated in Shanghai during 1998 pandemic period,were selected as represent strains in this research.Three recombinants were constructed based on 6 genes from F strain and HA,NA genes from SS strain by reverse genetic technique.RF7/SSHA virus was composed of the HA gene from SS strain and the remaining seven genes from F strain,RF7/SSNA virus was composed of the NA gene derived from SS strain and the remaining seven genes from F strain,and RF6/SSHA/SSNA virus was composed of the HA and NA genes of SS strain and the remaining 6 genes from F strain.Then the replicating property of the five H9N2 viruses were compared in SPF chickens.Groups of three 4-week old SPF chickens were inoculated with the AIVs by oral,tracheal and nasal routes.Virus replication was monitored 3 days after inoculation.Virus load in terms of ELD50/EID50 in trachea,lung and intestine were assayed 3 days after inoculation.The results showed that the replicating property of H9N2 subtype AIVs in chickens are determined by HA and NA genes mainly,and also related to internal genes.After 4 year's adaption and gene evolution,F strain isolated in 1998 have stronger replication ability than earlier AIV strain in respiratory system,which lay the foundation of aerosol infection. |
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| Keywords: | avian influenza virus H9N2 subtype replicating property reverse genetic tech nique HA gene NA gene |
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