| 不同营养水平日粮对陕北白绒山羊生长性能和小肠组织SLC7A7、SLC3A1及SLC15A1 mRNA表达的影响 |
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| 引用本文： | 曲星梅,邢晓南,薛复来,金妙函,彭孝坤,李佳腾,张恩平.不同营养水平日粮对陕北白绒山羊生长性能和小肠组织SLC7A7、SLC3A1及SLC15A1 mRNA表达的影响[J].畜牧兽医学报,2020,51(7):1656-1665. |
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| 作者姓名： | 曲星梅 邢晓南 薛复来 金妙函 彭孝坤 李佳腾 张恩平 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院, 杨凌 712100;2. 山东省诸城市畜牧兽医管理局, 诸城 262200 |
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| 基金项目： | 国家重点研发计划项目（2016YFD0500508）；陕西省重点研发计划项目饲料产业创新链（201TSCXL-NY-04-02）；陕西省农业科技创新转化项目（NYKJ-2016-04；NYKJ-2018-YL16） |
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| 摘 要： | 本试验旨在研究日粮营养水平对断奶后2~6月龄陕北白绒山羊生长性能及小肠组织中与氨基酸转运吸收相关的SLC7A7、SLC3A1和SLC15A1 mRNA表达的影响。选取健康、日龄（（60±1.60）d）和体重（（10.73±1.03）kg）相近的雌性陕北白绒山羊羔羊36只，随机分为4组，分别饲喂4种试验日粮，其消化能和粗蛋白质水平分别为标准日粮的85%、100%、115%和130%。标准日粮营养水平参考肉羊饲养标准NY/T816-2004，依据生长阶段（10~19 kg、15~27 kg）和目标增重设置。试验期间，分别于120和180日龄称重，于180日龄，每个重复屠宰1只试验羊，采集十二指肠中上部、空肠中段、回肠末端组织样品，通过实时荧光定量PCR检测SLC7A7、SLC3A1和SLC15A1表达水平。结果表明：1）第一阶段（60~120 d）115%水平组平均日增重显著高于其他三组（P<0.05），第二阶段（121~180 d）115%水平组平均日增重显著高于85%和100%水平组（P<0.05），与130%水平组无明显差异。两阶段115%水平组羔羊干物质采食量极显著高于其他3组（P<0.01）。两阶段料重比115%水平组显著低于85%、100%水平组（P<0.05）。2）相同营养水平下，SLC7A7和SLC15A1 mRNA的表达丰度顺序均为回肠>空肠>十二指肠；3）随日粮营养水平的增加，SLC7A7、SLC3A1和SLC15A1 mRNA在小肠各段的相对表达量呈先上升后下降的趋势，且115%水平组表达量最高。115%水平组SLC7A7和SLC15A1 mRNA相对表达量显著高于其他3组（P<0.05）；115%水平组SLC3A1 mRNA的相对表达量显著高于85%和130%水平组组（P<0.05）。本试验条件下，与其他营养水平组相比，115%水平组陕北白绒山羊羔羊在2~6月龄生长性能最佳，SLC7A7、SLC3A1和SLC15A1 mRNA在小肠各段的表达量最高。
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| 关 键 词： | 陕北白绒山羊 日粮营养水平 生长性能 SLC7A7 SLC3A1 SLC15A1 |
| 收稿时间： | 2019-11-29 |
Effects of Diets with Different Nutrition Levels on Growth Performance and Small Intestine Tissue SLC7A7、SLC3A1 and SLC15A1 mRNA Expression in Shaanbei White Cashmere Goats |
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| QU Xingmei,XING Xiaonan,XUE Fulai,JIN Miaohan,PENG Xiaokun,LI Jiateng,ZHANG Enping.Effects of Diets with Different Nutrition Levels on Growth Performance and Small Intestine Tissue SLC7A7、SLC3A1 and SLC15A1 mRNA Expression in Shaanbei White Cashmere Goats[J].Acta Veterinaria et Zootechnica Sinica,2020,51(7):1656-1665. |
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| Authors: | QU Xingmei XING Xiaonan XUE Fulai JIN Miaohan PENG Xiaokun LI Jiateng ZHANG Enping |
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| Institution: | 1. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China;2. Shandong Zhucheng Animal Husbandry and Veterinary Administration, Zhucheng 262200, China |
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| Abstract: | The aim of this study was to investigate the effects of diets with different nutrition levels on the growth performance and the mRNA expression of SLC7A7, SLC3A1 and SLC15A1 in intestinal tissues of 2-6 months old weaned Shaanbei white cashmere goats. Thirty-six healthy female Shaanbei white cashmere goats with age (60±1.60) d and body weight (10.73±1.03) kg were randomly divided into 4 groups and fed with 4 test diets, which digestive energy and crude protein were 85%, 100%, 115% and 130% of the standard diet, respectively. The nutrition level of standard diet formulated with reference to the NY/T816-2004. Goats were weighted at 120 and 180 d. One goat (a total of 12 goats) was slaughtered in each replicate. The upper middle part of the duodenum, the middle part of the jejunum and the ileum end tissue samples were collected. The mRNA expression levels of SLC7A7,SLC3A1 and SLC15A1 in the sample tissues were detected by real-time fluorescent quantitative PCR. The results showed that:1) In the first stage (60-120 d), the average daily gain of the 115% level group was significantly higher than those of other groups (P<0.05); In the second stage (121-180 d), the average daily gain of the 115% level group was significantly higher than 85% and 100% levels groups (P<0.05), but there was no significant difference compared to 130% level group (P>0.05). The dry matter intake of lambs in the 115% level group in the two stages were extremely significantly higher than those of other groups (P<0.01). The feed to gain ratio of 115% level group was significantly lower than those of 85% and 100% level groups (P<0.05). 2) With the same nutrition level, the mRNA expression levels of SLC7A7 and SLC15A1 were all in order of ileum>jejunum>duodenum; 3) The mRNA expression of SLC7A7, SLC3A1 and SLC15A1 in small intestines increased first and then decreased with the increase of dietary nutrient level, and the 115% level showed the highest expression. The mRNA expression of SLC7A7 and SLC15A1 in the 115% group was significantly higher than those of the the other three groups (P<0.05). The mRNA expression of SLC3A1 in the 115% group was significantly higher than those of the 85% and 130% groups (P<0.05). In this experiment, goats at age of 2-6 month-old fed the diet with 115% digestible energy and crude protein of the standard showed better growth performance, and the mRNA expression of SLC7A7, SLC3A1 and SLC15A1 in the small intestine was higher than those of the other groups. |
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| Keywords: | Shaanbei white cashmere goats dietary nutrition level growth performance SLC7A7 SLC3A1 SLC15A1 |
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