| 禽源结肠弯曲杆菌的耐药性分析与MLST分型 |
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| 引用本文： | 王新,周婷,孟江洪.禽源结肠弯曲杆菌的耐药性分析与MLST分型[J].畜牧兽医学报,2010,41(2). |
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| 作者姓名： | 王新 周婷 孟江洪 |
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| 作者单位： | 1. 西北农林科技大学食品学院,杨凌,712100
2. 西北农林科技大学动物医学院,杨凌,712100 |
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| 摘 要： | 为了解禽肉结肠弯曲杆菌的耐药表型和分子型,采用琼脂平板稀释法和多位点序列分型(Multilocus Se-quence Typing,MLST)技术分别对54株禽肉结肠弯曲杆菌进行耐药性及分子分型研究。耐药性试验结果得到单重耐药菌株有41株(75.9%),分别是对环丙沙星耐药有9株(16.7%)、对强力霉素耐药有23株(42.6%)和对红霉素耐药有9株(16.7%);多重耐药菌株有10株(18.5%),其中4株(7.4%)对环丙沙星和强力霉素耐药,1株(1.9%)对环丙沙星和红霉素耐药,4株(7.4%)对红霉素和强力霉素耐药,1株(1.9%)对环丙沙星、红霉素和强力霉素耐药;所有菌株对硫酸庆大霉素敏感。MLST得到了38个(含1个新的)等位基因(allele);26个(含8个新的)序列型(Sequence type,ST);2个已知序列型克隆系(ST clonal complexes),ST-828克隆系(45株,83.3%)和ST-1150克隆系(3株,5.6%),以及5个(6株,11.1%)没有序列型克隆系。耐药性与序列型和序列克隆系相关性比较,相关性不大。结果提示,禽肉结肠弯曲杆菌出现了对环丙沙星、红霉素和强力霉素的单重及多重耐药菌株;禽肉中结肠弯曲杆菌主要流行ST-828克隆系;耐药性与序列型及序列克隆系相关性差,揭示耐药菌株来源广泛。
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| 关 键 词： | 结肠弯曲杆菌 耐药性 多位点序列分型 基因型 |
Multilocus Sequence Typing for Molecular Typing of Campylobacter coli from Poultry Meat |
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| WANG Xin,ZHOU Ting,MENG Jiang-hong.Multilocus Sequence Typing for Molecular Typing of Campylobacter coli from Poultry Meat[J].Acta Veterinaria et Zootechnica Sinica,2010,41(2). |
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| Authors: | WANG Xin ZHOU Ting MENG Jiang-hong |
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| Institution: | WANG Xin1,ZHOU Ting2,MENG Jiang-hong1(1.College of Food Science , Engineering,Northwest A&F University,Yangling 712100,China,2.College of Veterinary Medicine,China) |
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| Abstract: | To determine the phenotypic and genotypic resistance of campylobacter coli(C.coli) isolates from poultry meat,54 C.coli isolates from retail poultry meat were phenotyped for ciprofloxacin(CIP),erythromycin(ERY),doxycycline(DOX) and gentamicin(GEN) by agar dilution method,and were genotyped by multilocus sequence typing(MLST).Within the 54 C.coli isolates,resistance only to CIP,ERY and DOX were respectively 16.7%,16.7% and 42.6%,18.5% of the isolates were resistant to two or three antimicrobial agents,wherea... |
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| Keywords: | C coli antibiotic resistance MLST genotype |
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