| 微量环丙沙星对人源大肠杆菌和粪肠球菌体外敏感性的影响 |
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| 引用本文： | 张可煜,王玉莲,胡丽霞,范盛先,袁宗辉.微量环丙沙星对人源大肠杆菌和粪肠球菌体外敏感性的影响[J].畜牧兽医学报,2006,37(3):286-290. |
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| 作者姓名： | 张可煜 王玉莲 胡丽霞 范盛先 袁宗辉 |
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| 作者单位： | 华中农业大学国家兽药残留基准实验室/农业部食品安全评价重点开放实验室,武汉,430070 |
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| 基金项目： | 国家自然科学基金资助项目（30271002）. |
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| 摘 要： | 建立人离体肠道模拟模型，研究微量环丙沙星对人源肠道大肠杆菌和粪肠球菌敏感性的影响，进而用聚合酶链反应法扩增耐药菌的gyrA基因的耐药决定区，并分析其耐药机制。结果显示，大肠杆菌连续培养后存活菌株对微量环丙沙星耐药，此耐药菌对其他抗菌药敏感；粪肠球菌绎连续培养，对环丙沙星和其他抗菌药物仍敏感；耐药大肠杆菌的gyrA基因发生突变，248位碱基由C变为T，259位由G变为T，相应地，该基因编码的蛋白质在83位的丝氨酸和87位的天冬氨酸分别改变为亮氨酸和酪氨酸。研究表明，微量环丙沙星对人肠道菌群具有不同的选择作用，能诱导大肠杆菌产生耐药性。这为动物源食品中环丙沙星残留的安全性评价提供了试验依据。
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| 关 键 词： | 环丙沙星 人源大肠杆菌 人源粪肠球菌 耐药性 |
| 文章编号： | 0366-6964（2006）03-0286-05 |
| 收稿时间： | 2004-12-23 |
| 修稿时间： | 2004-12-23 |
Effects of Ciprofloxacin on Sensitivity in vitro of Human E. coli and Enterococcus faecalis |
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| ZHANG Ke-yu,WANG Yu-lian,HU Li-xia,FAN Sheng-xian,YUAN Zong-hui.Effects of Ciprofloxacin on Sensitivity in vitro of Human E. coli and Enterococcus faecalis[J].Acta Veterinaria et Zootechnica Sinica,2006,37(3):286-290. |
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| Authors: | ZHANG Ke-yu WANG Yu-lian HU Li-xia FAN Sheng-xian YUAN Zong-hui |
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| Institution: | National Reference Laboratory of Veterinary Drug Residues and MOA Key Laboratory Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070,China |
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| Abstract: | An in vitro gut simulation model has been developed to study the effects of low doses of ciprofloxacin on human Escherichia coli and Enterococcus faecalis.The ciprofloxacin-resistance-determining regions(QRDR) of gyrA in the ciprofloxacin-resistant bacteria were amplified by polymerase chain reaction in order to study the mechanism of antibiotic resistance.The result showed that Escherichia coli could produce antibiotic resistance to ciprofloxacin,but not to other antibiotics.Enterococcus faecalis did not produce antibiotic resistance to all antibiotics.Those ciprofloxacin-resistant Escherichia coli strains had double mutations of C-to-T transition at position 248(Ser 83 to Leu) and G-to-T transition at position 259(Asp 87 to Tyr).The study demonstrated that low concentration of ciprofloxacin had selective pressure on human intestinal flora and could induce Escherichia coli to produce antibiotic resistance.It supplies the data for safety assessment on ciprofloxacin residues in foods of animal origin. |
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| Keywords: | ciprofloxacin human Escherichia coli human Enterococcus faecalis antibiotic resistance |
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